S17-1 lamp pir大肠杆菌克隆菌

基因型：

TpR SmR recA, thi, pro, hsdR-M+RP4： 2-Tc：Mu： Km Tn7 λpir.

基本信息：

抗性：带kan 无amp tet str

培养基：LB

菌株类别：大肠杆菌

培养条件：37℃，有氧，LB

质粒转化：42℃热激

保存方式：20%甘油，-20℃

基本应用：用于基因克隆

备注：S17-1 lamp pir是一种含pir基因的结合转移菌株。

菌株简介：

E. coli S17-1 λpir strain contains the pir gene (λpir). This strain have chromosomally integrated conjugal transfer functions (RP4 transfer functions), so when it is used as specific host strain into which the transposon vector DNA is transformed, the transfer occurs by biparental mating (a helper strain is not necessary).