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YEPlac112酿酒酵母质粒

货号：KL-ZL3327-3ug

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL3327-3ug	YEPlac112酿酒酵母质粒	3ug		立即咨询	+ -

产品介绍
相关产品

》产品信息

货号	名称	规格
KL-ZL3327	YEPlac112酿酒酵母质粒	3ug

》产品描述

原核抗性： Amp 筛选标记： TRP1 克隆菌株： DH5α 培养条件： LB/37℃

》产品简介

YEPlac112酿酒酵母质粒

》产品图谱及序列

序列：

CDS 3188..4048

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/note="AmpR"

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

rep_origin 4219..4807

/direction=RIGHT

/note="ori"

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

ORIGIN

1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca

61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct

121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat

181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc

241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg

301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac

361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac

421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt

481 gcggtatttc acaccgcata tatcggatcg tacttgttac ccatcattga attttgaaca

541 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat

601 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc

661 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc

721 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg

781 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa

841 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa

901 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa

961 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc

1021 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt

1081 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa

1141 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac

1201 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc

1261 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg

1321 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata

1381 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct

1441 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc

1501 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc

1561 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat

1621 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag

1681 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac

1741 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag

1801 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat

1861 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc

1921 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg

1981 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa

2041 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat

2101 aagctgtcaa acatgagaat tgatctttta tgcttgcttt tcaaaaggct tgcaggcaag

2161 tgcacaaaca atacttaaat aaatactact cagtaataac ctatttctta gcatttttga

2221 cgaaatttgc tattttgtta gagtctttta caccatttgt ctccacacct ccgcttacat

2281 caacaccaat aacgccattt aatctaagcg catcaccaac attttctggc gtcagtccac

2341 cagctaacat aaaatgtaag ctctcggggc tctcttgcct tccaacccag tcagaaatcg

2401 agttccaatc caaaagttca cctgtcccac ctgcttctga atcaaacaag ggaataaacg

2461 aatgaggttt ctgtgaagct gcactgagta gtatgttgca gtcttttgga aatacgagtc

2521 ttttaataac tggcaaaccg aggaactctt ggtattcttg ccacgactca tctccatgca

2581 gttggacgat atcaatgccg taatcattga ccagagccaa aacatcctcc ttaggttgat

2641 tacgaaacac gccaaccaag tatttcggag tgcctgaact atttttatat gcttttacaa

2701 gacttgaaat tttccttgca ataaccgggt caattgttct ctttctattg ggcacacata

2761 taatacccag caagtcagca tcggaatcta gtgcacattc tgcggcctct gtgctctgca

2821 agccgcaaac tttcaccaat ggaccagaac tacctgtgaa attaataaca gacatactcc

2881 aagctgcctt tgtgtgctta atcacgtata ctcacgtgct caatagtcac caatgccctc

2941 cctcttggcc ctctcctttt cttttttcga ccgaattaat tcttgaagac gaaagggcct

3001 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg

3061 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc

3121 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag

3181 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg

3241 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt

3301 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt

3361 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt

3421 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa

3481 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag

3541 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac

3601 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac

3661 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac

3721 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac

3781 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact

3841 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg

3901 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt

3961 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat

4021 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta

4081 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa

4141 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga

4201 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac

4261 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt

4321 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc

4381 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat

4441 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag

4501 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc

4561 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag

4621 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac

4681 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg

4741 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct

4801 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc

4861 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga

4921 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga

4981 agcggaaga

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