别名： pUB6-V5-His-LacZ
启动子： UBC
原核抗性： 氨苄青霉素Amp
筛选标记： 杀稻瘟菌素Blast
克隆菌株： 大肠杆菌DH5α
培养条件： 37℃
pUB6-V5-His-LacZ pUB6/V5-His /LacZ是一个哺乳细胞UBC启动子表达质粒。All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
序列：
ORIGIN
1 gacggatcgg gagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
61 cctcacggcg agcgctgcca cgtcagacga agggcgcagg agcgtcctga tccttccgcc
121 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
181 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
241 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
301 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
361 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
421 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
481 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
541 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
601 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
661 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
721 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
781 ttgcgggggc ggcagttatg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
841 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
901 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
961 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
1021 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
1081 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
1141 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
1201 tggccgtttt tggctttttt gttagacgaa gcttggtacc gagctcggat ccactagtcc
1261 agtgtggtgg aattctgcag atcgaaacga tgatagatcc cgtcgtttta caacgtcgtg
1321 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
1381 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
1441 atggcgaatg gcgctttgcc tggtttccgg taccagaagc ggtgccggaa agctggctgg
1501 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt
1561 acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg ccgtttgttc
1621 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac
1681 aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca
1741 acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg
1801 catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg agtgacggca
1861 gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc
1921 tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt
1981 tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac
2041 gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg
2101 gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg
2161 tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac
2221 tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg
2281 tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta
2341 accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg
2401 atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc
2461 atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata
2521 ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg
2581 cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca
2641 tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga
2701 tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg
2761 ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg
2821 tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc
2881 tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg
2941 cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc
3001 tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata
3061 cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc
3121 cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa
3181 ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga
3241 tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac
3301 aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct
3361 ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca
3421 gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc
3481 acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc
3541 gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac
3601 aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg
3661 taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc
3721 attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc
3781 tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa
3841 cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg
3901 atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg
3961 taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt
4021 ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa
4081 acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact
4141 tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc
4201 tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg
4261 gcgacgactc ctggagcccg tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc
4321 attaccagtt ggtctggtgt caaaaagcgg ccgctcgagg tcacccattc gaaggtaagc
4381 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc
4441 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
4501 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
4561 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
4621 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
4681 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
4741 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
4801 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
4861 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta
4921 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
4981 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
5041 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
5101 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
5161 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
5221 aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
5281 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
5341 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
5401 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
5461 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
5521 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
5581 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt
5641 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
5701 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
5761 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
5821 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
5881 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
5941 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
6001 ctctggttat gtgtgggagg gctaagcact tcgtggccga ggagcaggac tgacacgtgc
6061 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
6121 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
6181 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
6241 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
6301 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
6361 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
6421 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
6481 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
6541 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
6601 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
6661 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
6721 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
6781 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
6841 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
6901 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
6961 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
7021 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
7081 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
7141 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
7201 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
7261 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
7321 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
7381 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
7441 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
7501 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
7561 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
7621 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
7681 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
7741 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
7801 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
7861 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
7921 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
7981 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
8041 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
8101 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
8161 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
8221 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
8281 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
8341 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
8401 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
8461 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
