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pRL-CMV质粒

  • 货号:kl-zl-1019-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-1019-01 pRL-CMV质粒 NA

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pRL-CMV质粒 货号:kl-zl-1019 规格:20ul

启动子: CMV,T7
复制子: pUC ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳细胞,信号通路报告载体
质粒大小: 4079bp
原核抗性: Amp
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'测序引物: Sv40-polyA-R:GAAATTTGTGATGCTATTGC
质粒简介:
The pRL-CMV Vector may be used in combination with any experimental reporter vector to co-transfect mammalian cells. However, it is important to realize that trans effects between promoters on co-transfected plasmids can potentially affect reporter gene expression . Primarily, this is of concern when either the control or experimental reporter vector, or both, contain very strong promoter/enhancer elements (such as CMV). The occurrence and magnitude of such effects will depend on several factors: a) the combination and activities of the genetic regulatory elements present on the co-transfected vectors, b) the relative ratio of experimental vector to control vector introduced into the cells, and c) the cell type transfected.
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