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pTRV2质粒

货号：kl-zl-0790-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0790-01	pTRV2质粒	NA		立即咨询	+ -

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pTRV2质粒货号：kl-zl-0790 规格：20ul

启动子：	CaMV 35S
复制子：	pVS1 oriV，ori
质粒分类：	植物系列，RNAi载体
质粒大小：	9663bp
原核抗性：	Kan
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	植物细胞
5'测序引物：	35S：GACGCACAATCCCACTATCC
3'测序引物：	根据序列设计引物

质粒简介：
Tobacco rattle virus (TRV) is a bipartite, positive-sense RNA virus. TRV1 (i.e., RNA1) encodes 134 and 194 kDa replicase proteins from the genomic RNA,29 kDa movement protein, and16 kDa cysteine-rich proteinfrom subgenomic RNAs. TRV1 can replicate and move systemically without TRV2 (i.e., RNA2). TRV2 encodescoat proteinfrom the genomic and two nonstructural proteins from subgenomic RNAs (A). To develop the TRV-based VIGS vector, a cDNA clone of TRV1 or TRV2 (Ppk20 strain) was placed between a duplicated CaMV 35S promoter (2X35S) and nopaline synthase terminator (NOSt) in an Agrobacterium T-DNA vector. In the TRV2 cDNA construct, the nonessential structural genes were replaced with MCS for cloning the target gene sequences. Further, a self-cleaving ribozyme site was included at the 3' end of TRV2. The TRV1 (AF406990)-based viral vector, pTRV1 (linear plasmid 6.791 kb), is used along with pTRV2 for silencing. The pTRV2 has three variants. The first one is suitable for conventional cloning (AF406991, linear plasmid 9663 nucleotide). The second variant is a gateway-cloning-compatible vector. The third variant is suitable for ligation-independent cloning. Rz, self-cleaving ribozyme; MCS, multiple cloning site;CP,coat protein;MP,movement protein. These vectors were created by Dr. S.P. Dinesh-Kumar, UC Davis, USA. Conventional cloning-compatible (YL156) and ligation-independent-compatible (YY13TRV2) vector plasmid DNA can be obtained from ABRC, Stock No. CD3-1040 vector name YL156. Ligation-independent-compatible TRV2 vector plasmid DNA is ABRC Stock No. CD3-1042, vector name YY13.
质粒图谱：

序列：

ORIGIN

1 ataaaacatt gcacctatgg tgttgccctg gctggggtat gtcagtgatc gcagtagaat

61 gtactaattg acaagttgga gaatacggta gaacgtcctt atccaacaca gcctttatcc

121 ctctccctga cgaggttttt gtcagtgtaa tatttctttt tgaactatcc agcttagtac

181 cgtacgggaa agtgactggt gtgcttatct ttgaaatgtt actttgggtt tcggttcttt

241 aggttagtaa gaaagcactt gtcttctcat acaaaggaaa acctgagacg tatcgcttac

301 gaaagtagca atgaaagaaa ggtggtggtt ttaatcgcta ccgcaaaaac gatggggtcg

361 ttttaattaa cttctcctac gcaagcgtct aaacggacgt tggggttttg ctagtttctt

421 tagagaaaac tagctaagtc tttaatgtta tcattagaga tggcataaat ataatacttg

481 tgtctgctga taagatcatt ttaatttgga cgattagact tgttgaacta caggttactg

541 aatcacttgc gctaatcaac atgggagata tgtacgatga atcatttgac aagtcgggcg

601 gtcctgctga cttgatggac gattcttggg tggaatcagt ttcgtggaaa gatctgttga

661 agaagttaca cagcataaaa tttgcactac agtctggtag agatgagatc actgggttac

721 tagcggcact gaatagacag tgtccttatt caccatatga gcagtttcca gataagaagg

781 tgtatttcct tttagactca cgggctaaca gtgctcttgg tgtgattcag aacgcttcag

841 cgttcaagag acgagctgat gagaagaatg cagtggcggg tgttacaaat attcctgcga

901 atccaaacac aacggttacg acgaaccaag ggagtactac tactaccaag gcgaacactg

961 gctcgacttt ggaagaagac ttgtacactt attacaaatt cgatgatgcc tctacagctt

1021 tccacaaatc tctaacttcg ttagagaaca tggagttgaa gagttattac cgaaggaact

1081 ttgagaaagt attcgggatt aagtttggtg gagcagctgc tagttcatct gcaccgcctc

1141 cagcgagtgg aggtccgata cgtcctaatc cctagggatt taaggacgtg aactctgttg

1201 agatctctgt gaaattcaga gggtgggtga taccatattc actgatgcca ttagcgacat

1261 ctaaataggg ctaattgtga ctaatttgag ggaatttcct ttaccattga cgtcagtgtc

1321 gttggtagca tttgagtttc gcaatgcacg aattacttag gaagtggctt gacgacacta

1381 atgtgttatt gttagataat ggtttggtgg tcaaggtacg tagtagagtc ccacatattc

1441 gcacgtatga agtaattgga aagttgtcag tttttgataa ttcactggga gatgatacgc

1501 tgtttgaggg aaaagtagag aacgtatttg tttttatgtt caggcggttc ttgtgtgtca

1561 acaaagatgg acattgttac tcaaggaagc acgatgagct ttattattac ggacgagtgg

1621 acttagattc tgtgagtaag gttaccgaat tctctagaag gcctccatgg ggatccggta

1681 ccgagctcac gcgtctcgag gcccgggcat gtcccgaaga cattaaacta cggttcttta

1741 agtagatccg tgtctgaagt tttaggttca atttaaacct acgagattga cattctcgac

1801 tgatcttgat tgatcggtaa gtcttttgta atttaatttt ctttttgatt ttattttaaa

1861 ttgttatctg tttctgtgta tagactgttt gagatcggcg tttggccgac tcattgtctt

1921 accatagggg aacggacttt gtttgtgttg ttattttatt tgtattttat taaaattctc

1981 aacgatctga aaaagcctcg cggctaagag attgttgggg ggtgagtaag tacttttaaa

2041 gtgatgatgg ttacaaaggc aaaaggggta aaacccctcg cctacgtaag cgttattacg

2101 cccgtctgta cttatatcag tacactgacg agtccctaaa ggacgaaacg ggagaacgct

2161 agccaccacc accaccacca cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg

2221 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat

2281 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac

2341 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat

2401 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt

2461 actagatcgg gaattaaact atcagtgttt gacaggatat attggcgggt aaacctaaga

2521 gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc

2581 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc

2641 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa

2701 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc

2761 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac

2821 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg

2881 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt

2941 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc

3001 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc

3061 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg

3121 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg

3181 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg

3241 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc

3301 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg

3361 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg

3421 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg

3481 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga

3541 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga

3601 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc

3661 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa

3721 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg

3781 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag

3841 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc

3901 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc

3961 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac

4021 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac

4081 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct

4141 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg

4201 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc

4261 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg

4321 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc

4381 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt

4441 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc

4501 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga

4561 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac

4621 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa

4681 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat

4741 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg

4801 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc

4861 gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg

4921 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc

4981 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg

5041 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc

5101 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga

5161 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga

5221 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga

5281 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga

5341 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt

5401 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt

5461 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac

5521 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg

5581 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa

5641 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg

5701 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa

5761 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg

5821 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc

5881 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc

5941 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg

6001 tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa

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