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pBIFC-bFosVC155质粒

货号：kl-zl-1006-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1006-01	pBIFC-bFosVC155质粒	NA		立即咨询	+ -

产品介绍
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pBIFC-bFosVC155质粒货号：kl-zl-1006 规格：20ul

启动子：	CMV
复制子：	pUC ori
终止子：	SV40 poly(A) signal
质粒分类：	哺乳细胞，双分子荧光互补载体
质粒大小：	4352bp
原核抗性：	Amp
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物：	根据序列设计引物

质粒简介：
Bimolecular fluorescence complementation (BiFC) analysis enables direct visualization of protein interactions in living cells. The BiFC assay is based on the discoveries that two non-fluorescent fragments of a fluorescent protein can form a fluorescent complex and that the association of the fragments can be facilitated when they are fused to two proteins that interact with each other. BiFC must be confirmed by parallel analysis of proteins in which the interaction interface has been mutated. The BiFC assay has been used for the visualization of interactions between many types of proteins in different subcellular locations and in different cell types and organisms. It is technically straightforward and can be performed using a regular fluorescence microscope and standard molecular biology and cell culture reagen
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