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pACT-MyoD质粒

  • 货号:kl-zl-0994-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0994-01 pACT-MyoD质粒 NA

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pACT-MyoD质粒 货号:kl-zl-0994 规格:20ul

启动子: T7,CMV
复制子: pUC ori,f1 ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳细胞,哺乳细胞双杂交载体
质粒大小: 7400bp
原核抗性: Amp
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'测序引物: 根据序列设计引物
质粒简介:
The CheckMate™ Mammalian Two-Hybrid System provides positive control vectors that encode and express two proteins known to interact in vivo (13). MyoD is expressed in skeletal muscle and is a myogenic regulatory protein (14,15). The Id protein acts as a negative regulator of myogenic differentiation (16). MyoD and Id are members of the helix-loop-helix family of nuclear proteins. The pBIND-Id Control Vector contains a segment of murine Id cDNA (amino acids 29–148; 13,16) cloned in-frame with the GAL4 DNA-binding domain of the pBIND Vector . The pACT-MyoD Control Vector contains a segment of murine MyoD cDNA (amino acids 1–318; 13,15) cloned in-frame with the VP16 activation domain of the pACT Vector .
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