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p53-ABAI质粒

  • 货号:kl-zl-0561-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0561-01 p53-ABAI质粒 NA

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p53-ABAI质粒 货号:kl-zl-0561 规格:20ul

启动子: URA3
复制子: pUC ori
质粒分类: 酵母系列,酵母单杂交载体
质粒大小: 4944bp
原核抗性: Amp
筛选标记: URA3
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 酵母细胞
5'测序引物: 根据序列设计引物
3'测序引物: 根据序列设计引物
质粒简介:
p53-AbAi is a yeast reporter vector that serves as a positive control in the Matchmaker Gold Yeast One-Hybrid Library Screening System. The vector contains a p53 binding site, located upstream of the yeast iso-1-cytochrome C minimal promoter and the AUR1-C gene, an antibiotic resistance gene that confers resistance to Aureobasidin A. Expression of AUR1-C, and thus AbA resistance, is induced by the binding of GAL4 activation domain-p53 fusion proteins to the p53 binding site upstream of AUR1-C. p53-AbAi cannot be propagated episomally in yeast; it can only be stably maintained through integration into the host genome. Integration is accomplished via homologous recombination between the vector’s URA3 gene and the nonfunctional ura3-52 locus of the yeast strain provided in the Matchmaker Gold Yeast One-Hybrid System. URA3 is a nutritional marker that can also be used for the selection of recombinant yeast.
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