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pMIR-Reporter质粒

货号：kl-zl-1015-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1015-01	pMIR-Reporter质粒	NA		立即咨询	+ -

产品介绍
相关产品

pMIR-Reporter质粒货号：kl-zl-1015 规格：20ul

启动子：	CMV,SP6,T7,Lac
复制子：	pUC ori，SV40 ori
终止子：	SV40 poly(A) signal
质粒分类：	哺乳细胞，信号通路报告载体
质粒大小：	6470bp
原核抗性：	Amp
筛选标记：	Puro
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物：	M13F：TGTAAAACGACGGCCAGT

质粒简介：
The pMIR-REPORT™ miRNA Expression Reporter Vector System consists of an experimental firefly luciferase reporter vector and an associated β-gal reporter control plasmid. By inserting predicted miRNA target sequences in the multiple cloning site, pMIR-REPORT Luciferase miRNA Expression Reporter Vector can be used to conduct accurate, quantitative evaluations of miRNA function. It can also be used to evaluate siRNA target sites and to analyze the influence of 3' UTR sequences on gene expression. pMIR-REPORT Luciferase contains a firefly luciferase reporter gene under the control of a CMV promoter/termination system. The 3' UTR of the luciferase gene contains a multiple cloning site for insertion of predicted miRNA binding targets or other nucleotide sequences. By cloning a predicted miRNA target sequence into pMIR-REPORT, the luciferase reporter is subjected to regulation that mimics the miRNA target.
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