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pETBlue-2质粒

  • 货号:KL-ZL-0127-01
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL-0127-01 pETBlue-2质粒 NA

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产品名称:pETBlue-2 质粒

货号:KL-ZL-0127


价格:询价

详细描述:

启动子: T7/lac
复制子: ColE1 ori,F1 ori
终止子: T7 terminator
质粒分类: 大肠杆菌载体;PET系列表达质粒
质粒大小: 3653bp
质粒标签: C-HSV,C-6×His
原核抗性: 氨苄青霉素Amp
克隆菌株: DH5α
培养条件: 37℃,有氧,LB
表达宿主: BL21(DE3)
诱导方式: IPTG或乳糖及其类似物
5'测序引物: T7:TAATACGACTCACTATAGGG
3'测序引物: T7-ter:TGCTAGTTATTGCTCAGCGG

产地:美国,中国

运输方式:冻存运输
质粒简介:
The pETBlue™-2 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-2 defines the open reading frame and inserts must be cloned in-frame if expression is desired. The vector features an expanded multiple cloning site (MCS) and optional C-terminal HSV•Tag® and His•Tag® sequences. The sequence is numbered from the first base of the T7 promoter sequence.
质粒图谱:

启动子 T7/lac
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