pET-24a质粒

产品名称：pET-24a质粒 货号：KL-ZL0055

一：质粒简介

别称：	pET24a
启动子：	T7
复制子：	pBR322
终止子：	T7 terminator
质粒分类：	大肠杆菌载体；PET系列表达质粒
质粒大小：	5310bp
质粒标签：	N-T7, C-6×His
原核抗性：	Kan
克隆菌株：	DH5a
培养条件：	37度
表达宿主：	大肠杆菌BL21(DE3)
培养条件：	37℃，有氧，LB
诱导方式：	IPTG或乳糖及其类似物
5'测序引物：	T7：TAATACGACTCACTATAGGG
3'测序引物：	T7-ter：TGCTAGTTATTGCTCAGCGG

The pET-24a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs. ampicillin resistance). Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, singlestranded sequencing should be performed using the T7 terminator primer .

二，图谱序列：