pGL4.22[luc2CP/Puro]哺乳报告质粒

复制子： pUC 原核抗性： 氨苄青霉素Amp 筛选标记： 嘌呤霉素Puro 克隆菌株： 大肠杆菌DH5α 培养条件： 37℃

pGL4.22[luc2CP/Puro]是一个哺乳细胞单荧光素酶报告质粒。The pGL4.22[luc2CP/Puro] Vector encodes the luciferase reporter gene luc2CP (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector contains a mammalian selectable marker for puromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The vector is engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression. The pGL4.22[luc2CP/Puro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The luc2CP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by luc2CP responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.

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