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pECMV-3×Flag-T7 RNA polymerase聚合酶基因质粒

货号：KL-ZL3363-3ug

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL3363-3ug	pECMV-3×Flag-T7 RNA polymerase聚合酶基因质粒	3ug		立即咨询	+ -

产品介绍
相关产品

》产品信息

货号	名称	规格
KL-ZL3363	pECMV-3×Flag-T7 RNA polymerase聚合酶基因质粒	3ug

》产品描述

原核抗性：氨苄青霉素Amp 筛选标记：遗传霉素G418 克隆菌株：大肠杆菌DH5α 培养条件： 37℃

》产品简介

pECMV-3×FLAG-T7 RNA polymerase是一个哺乳细胞T7 RNA聚合酶表达质粒。

》产品图谱及序列

序列：

CDS complement(7138..7998)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=Amp

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(7999..8103)

/gene="bla"

/label=AmpR promoter

ORIGIN

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc

901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac

961 atcgactaca aggatgacga tgacaagctt ggtaccgagc tcggatccac tagtccagtg

1021 tggtggaatt ctatgaacac gattaacatc gctaagaacg acttctctga catcgaactg

1081 gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag

1141 ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag

1201 cgtcaactta aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc

1261 ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc

1321 ggcaagcgcc cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac

1381 atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct

1441 gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac

1501 cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac

1561 gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc

1621 ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc

1681 atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta

1741 gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc

1801 cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag

1861 ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg

1921 gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg

1981 tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg

2041 gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag

2101 cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg

2161 tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc

2221 agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc

2281 ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt

2341 aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt

2401 tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct

2461 gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca

2521 ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt

2581 gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt

2641 gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt

2701 cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag

2761 aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga agtagttacc

2821 gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg

2881 gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg

2941 ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag

3001 ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac

3061 atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg

3121 aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga

3181 gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg

3241 caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc

3301 ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct

3361 ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg

3421 tgggcacacg agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc

3481 attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat

3541 gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct

3601 caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta

3661 gagtcggact tcgcgttcgc gttcgcgtaa ctcgagtcta gagggcccgt ttaaacccgc

3721 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg

3781 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt

3841 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc

3901 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct

3961 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc

4021 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc

4081 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc

4141 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc

4201 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg

4261 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact

4321 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt

4381 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt

4441 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc

4501 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag

4561 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc

4621 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa

4681 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt

4741 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca

4801 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat

4861 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac

4921 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc

4981 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc

5041 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag

5101 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc

5161 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg

5221 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc

5281 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc

5341 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga

5401 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca

5461 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg

5521 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg

5581 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg

5641 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga

5701 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg

5761 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat

5821 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt

5881 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg

5941 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg

6001 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc

6061 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt

6121 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg

6181 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt

6241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc

6301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa

6361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa

6421 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc

6481 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc

6541 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag

6601 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga

6661 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc

6721 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac

6781 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg

6841 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca

6901 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg

6961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc

7021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa

7081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta

7141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt

7201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag

7261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca

7321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc

7381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt

7441 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag

7501 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt

7561 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat

7621 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt

7681 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc

7741 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat

7801 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag

7861 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt

7921 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg

7981 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta

8041 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc

8101 gcgcacattt ccccgaaaag tgccacctga cgtc

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