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pRK5-HA哺乳表达质粒

货号：KL-ZL3357-3ug

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL3357-3ug	pRK5-HA哺乳表达质粒	3ug		立即咨询	+ -

产品介绍
相关产品

》产品信息

货号	名称	规格
KL-ZL3357	pRK5-HA哺乳表达质粒	3ug

》产品描述

原核抗性：氨苄青霉素Amp 克隆菌株：大肠杆菌DH5α 培养条件： 37℃

》产品简介

pRK5-HA是一个哺乳细胞过表达质粒。

》产品图谱及序列

序列：

CDS 2690..3550

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

rep_origin 3721..4310

/direction=RIGHT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

ORIGIN

1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca

61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct

121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta

181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac

241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt

301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag

361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat

421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat

481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc

541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt

601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga

661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt

721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta

781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac

841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc

901 acctcggttc tatcgattga attcccgaac cgacagtcgg tctcttcacc aaggccattc

961 gcgccaccat gggctacccc tatgatgtgc ctgactacgc agatctcaat ggtggtggtg

1021 gtgggtcgac gtacccgggc gtcaagcttg ctagatatcc gcatgcggcc gctaagtaag

1081 taaggatccc cagcttggcc gccatggccc aacttgttta ttgcagctta taatggttac

1141 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt

1201 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcgggaa ttaattcggc

1261 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc

1321 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca

1381 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta

1441 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga

1501 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag

1561 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctgttaac agcttggcac

1621 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc

1681 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc

1741 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta

1801 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag

1861 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag

1921 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt

1981 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca

2041 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata

2101 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca

2161 aactggaaca acactcaacc ctatctcggg ctattctttt aatttataag ggattttgcc

2221 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa

2281 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc

2341 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct

2401 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag

2461 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt

2521 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa

2581 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat

2641 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca

2701 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca

2761 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta

2821 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt

2881 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc

2941 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc

3001 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc

3061 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa

3121 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga

3181 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat

3241 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca

3301 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc

3361 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat

3421 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag

3481 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa

3541 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca

3601 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc

3661 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc

3721 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc

3781 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt

3841 cagcagagcg cagataacca aatactgttc ttctagtgta gccgtagtta ggccaccact

3901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg

3961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata

4021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga

4081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag

4141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg

4201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac

4261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca

4321 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg

4381 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc

4441 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa

4501 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt

4561 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt

4621 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg

4681 gataacaatt tcacacagga aacagctatg acatgattac gaattaa

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