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pCDNA3.1-EEF2-Myc-6×His人源基因质粒

  • 货号：KL-ZL3337-3ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL3337-3ug pCDNA3.1-EEF2-Myc-6×His人源基因质粒 3ug

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》产品信息

货号 名称 规格
KL-ZL3337 pCDNA3.1-EEF2-Myc-6×His人源基因质粒

3ug

》产品描述

别名： NM_001961.3 启动子： CMV 复制子： pUC 终止子： bGH 原核抗性： 氨苄青霉素Amp 筛选标记： 遗传霉素G418 克隆菌株： 大肠杆菌DH5α 培养条件： 37℃

》产品简介

pCDNA3.1-EEF2-Myc-6×His(NM_001961.3)是一个人源基因哺乳细胞过表达质粒。

》产品图谱及序列


序列：
CDS complement(7089..7949)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7950..8054)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcg
961 ccaccatggt gaacttcacg gtagaccaga tccgcgccat catggacaag aaggccaaca
1021 tccgcaacat gtctgtcatc gcccacgtgg accatggcaa gtccacgctg acagactccc
1081 tggtgtgcaa ggcgggcatc atcgcctcgg cccgggccgg ggagacacgc ttcactgata
1141 cccggaagga cgagcaggag cgttgcatca ccatcaagtc aactgccatc tccctcttct
1201 acgagctctc ggagaatgac ttgaacttca tcaagcagag caaggacggt gccggcttcc
1261 tcatcaacct cattgactcc cccgggcatg tcgacttctc ctcggaggtg actgctgccc
1321 tccgagtcac cgatggcgca ttggtggtgg tggactgcgt gtcaggcgtg tgcgtgcaga
1381 cggagacagt gctgcggcag gccattgccg agcgcatcaa gcctgtgctg atgatgaaca
1441 agatggaccg cgccctgctg gagctgcagc tggagcccga ggagctctac cagactttcc
1501 agcgcatcgt ggagaacgtg aacgtcatca tctccaccta cggcgagggc gagagcggcc
1561 ccatgggcaa catcatgatc gatcctgtcc tcggtaccgt gggctttggg tctggcctcc
1621 acgggtgggc cttcaccctg aagcagtttg ccgagatgta tgtggccaag ttcgccgcca
1681 agggggaggg ccagttgggg cctgccgagc gggccaagaa agtagaggac atgatgaaga
1741 agctgtgggg tgacaggtac tttgacccag ccaacggcaa gttcagcaag tcagccacca
1801 gccccgaagg gaagaagctg ccacgcacct tctgccagct gatcctggac cccatcttca
1861 aggtgtttga tgcgatcatg aatttcaaga aagaggagac agcaaaactg atagagaaac
1921 tggacatcaa actggacagc gaggacaagg acaaagaagg caaacccctg ctgaaggctg
1981 tgatgcgccg ctggctgcct gccggagacg ccttgttgca gatgatcacc atccacctgc
2041 cctcccctgt gacggcccag aagtaccgct gcgagctcct gtacgagggg cccccggacg
2101 acgaggctgc catgggcatt aaaagctgtg accccaaagg ccctcttatg atgtatattt
2161 ccaaaatggt gccaacctcc gacaaaggtc ggttctacgc ctttggacga gtcttctcgg
2221 ggctggtctc cactggcctg aaggtcagga tcatggggcc caactatacc cctgggaaga
2281 aggaggacct ctacctgaag ccaatccaga gaacaatctt gatgatgggc cgctacgtgg
2341 agcccatcga ggatgtgcct tgtgggaaca ttgtgggcct cgtgggcgtg gaccagttcc
2401 tggtgaagac gggcaccatc accaccttcg agcacgcgca caacatgcgg gtgatgaagt
2461 tcagcgtcag ccctgttgtc agagtggccg tggaggccaa gaacccggct gacctgccca
2521 agctggtgga ggggctgaag cggctggcca agtccgaccc catggtgcag tgcatcatcg
2581 aggagtcggg agagcatatc atcgcgggcg ccggcgagct gcacctggag atctgcctga
2641 aggacctgga ggaggaccac gcctgcatcc ccatcaagaa atctgacccg gtcgtctcgt
2701 accgcgagac ggtcagtgaa gagtcgaacg tgctctgcct ctccaagtcc cccaacaagc
2761 acaaccggct gtacatgaag gcgcggccct tccccgacgg cctggccgag gacatcgata
2821 aaggcgaggt gtccgcccgt caggagctca agcagcgggc gcgctacctg gccgagaagt
2881 acgagtggga cgtggctgag gcccgcaaga tctggtgctt tgggcccgac ggcaccggcc
2941 ccaacatcct caccgacatc accaagggtg tgcagtacct caacgagatc aaggacagtg
3001 tggtggccgg cttccagtgg gccaccaagg agggcgcact gtgtgaggag aacatgcggg
3061 gtgtgcgctt cgacgtccac gacgtcaccc tgcacgccga cgccatccac cgcggagggg
3121 gccagatcat ccccacagca cggcgctgcc tctatgccag tgtgctgacc gcccagccac
3181 gcctcatgga gcccatctac cttgtggaga tccagtgtcc agagcaggtg gtcggtggca
3241 tctacggggt tttgaacagg aagcggggcc acgtgttcga ggagtcccag gtggccggca
3301 cccccatgtt tgtggtcaag gcctatctgc ccgtcaacga gtcctttggc ttcaccgctg
3361 acctgaggtc caacacgggc ggccaggcgt tcccccagtg tgtgtttgac cactggcaga
3421 tcctgcccgg agaccccttc gacaacagca gccgccccag ccaggtggtg gcggagaccc
3481 gcaagcgcaa gggcctgaaa gaaggcatcc ctgccctgga caacttcctg gacaaattgg
3541 gatccgagct cggtaccaag cttgggcccg aacaaaaact catctcagaa gaggatctga
3601 atagcgccgt cgaccatcat catcatcatc attgagttta aacggtctcc agcttaagtt
3661 taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
3721 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
3781 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
3841 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
3901 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
3961 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
4021 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
4081 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
4141 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
4201 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
4261 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
4321 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
4381 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
4441 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
4501 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
4561 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
4621 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
4681 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
4741 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
4801 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
4861 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
4921 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
4981 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
5041 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
5101 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
5161 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
5221 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
5281 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
5341 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
5401 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
5461 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
5521 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
5581 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
5641 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
5701 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
5761 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
5821 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
5881 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
5941 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
6001 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
6061 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
6121 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
6181 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
6241 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
6301 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
6361 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
6421 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
6481 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
6541 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
6601 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
6661 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
6721 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
6781 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
6841 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
6901 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
6961 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
7021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
7081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
7141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
7201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
7261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
7321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
7381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
7441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
7501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
7561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
7621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
7681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
7741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
7801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
7861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
7921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
7981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
8041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc

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