| 货号 | 产品名称 | 规格 | 库存 | 价格 | 数量 | 购买 |
|---|---|---|---|---|---|---|
| C0131 | 永生化人脑微血管内皮细胞hCMEC/D3 | 支 |
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| 英文名称 | HCMEC/D3; CMEC/D3; human Cortical Microvessels Endothelial Cells/D3 |
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| 建系背景 | The hCMEC/D3 cell line was derived from human temporal lobe microvessels isolated from tissue excised during surgery for control of epilepsy. The primary isolate was enriched in cerebral endothelial cells (CECs). In the first passage, cells were sequentially immortalized by lentiviral vector transduction with the catalytic subunit of human telomerase (hTERT) and SV40 large T antigen, following which CEC were selectively isolated by limited dilution cloning, and clones were extensively characterized for brain endothelial phenotype. This brain microvascular endothelial cell line represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS). |
| 组织来源 | 脑微血管 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 细胞培养条件 | ECM内皮专用培养基 |
| 培养条件气体 | 空气，95%；CO2，5%；温度：37℃ |
| 推荐冻存条件 | 30% 基础培养基+60%FBS+10%DMSO |
| 冻存液氮 | 液氮保存 |
| 推荐传代比例 | 1：3--1：5 |
| 推荐换液频率 | 2-3次/周 |
| 货号 | 产品名称 | 规格 | 库存 | 价格 | 数量 | 购买 |
|---|---|---|---|---|---|---|
| C0401 | 小鼠肾小管上皮细胞TCMK-1 | 1支 |
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| C0262 | 小鼠肺成纤维细胞永生化 | 1支 |
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| C0092 | 大鼠神经雪旺氏细胞/大鼠神经鞘瘤细胞/大鼠神经许旺细胞RT4-D6P2T | 1支 |
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| C0181 | 人胚肺成纤维细胞HFL-1 | 1支 |
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| C0344 | 人慢性髓原白血病细胞K562 | 1支 |
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| C0431 | 小鼠胚胎成骨细胞前体细胞MC3T3-E1 | 1支 |
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| C0088 | 大鼠肾细胞NRK-52E | 1支 |
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| C0278 | 中国仓鼠卵巢细胞CHO | 1支 |
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| C0265 | 大鼠肝星状细胞永生化 | 1支 |
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| C0145 | 小鼠卵巢癌上皮细胞ID8 | 1支 |
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| C0090 | 大鼠肝细胞BRL-3A | 1支 |
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| C0017 | 人正常结肠上皮细胞NCM460 | 1支 |
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