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人弥漫大B淋巴瘤细胞U-2932

  • 货号:C0130
货号 产品名称 规格 库存 价格 数量 购买
C0130 人弥漫大B淋巴瘤细胞U-2932

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英文名称 U2932
建系背景 established in 1996 from the ascites of a 29-year-old woman with diffuse large B cell lymphoma, who 16 years earlier was diagnosed with advanced stage Hodgkin lymphoma, and relapsed several times after multiple chemo- and radiotharapy regimens to complete remissions; cells were described in the literature to overexpress BCL2, BCL6 and p53; assigned to ABC-like lymphoma subtype (activated B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq). Also, please note: the cell line U-2932 harbours two distinct subpopulations as visible by surface staining of CD19, CD20, and CD38 (see ref 18133); continuous monitoring of subpopulations is advised to prevent accidental loss during passaging
组织来源 腹水
生长特性 悬浮细胞
细胞形态 淋巴母细胞样,圆形,单个细胞,少数聚团
细胞培养条件 RPMI-1640+10% FBS+1% P/S
培养条件气体 空气,95%;CO2,5%;温度:37℃
推荐冻存条件 30% 基础培养基+60%FBS+10%DMSO
冻存液氮 液氮保存
推荐传代比例 1:3--1:4
推荐换液频率 2-3次/周
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