
| 货号 | 产品名称 | 规格 | 库存 | 价格 | 数量 | 购买 |
|---|---|---|---|---|---|---|
| C0130 | 人弥漫大B淋巴瘤细胞U-2932 | 支 |
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| 英文名称 | U2932 |
|---|---|
| 建系背景 | established in 1996 from the ascites of a 29-year-old woman with diffuse large B cell lymphoma, who 16 years earlier was diagnosed with advanced stage Hodgkin lymphoma, and relapsed several times after multiple chemo- and radiotharapy regimens to complete remissions; cells were described in the literature to overexpress BCL2, BCL6 and p53; assigned to ABC-like lymphoma subtype (activated B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq). Also, please note: the cell line U-2932 harbours two distinct subpopulations as visible by surface staining of CD19, CD20, and CD38 (see ref 18133); continuous monitoring of subpopulations is advised to prevent accidental loss during passaging |
| 组织来源 | 腹水 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴母细胞样,圆形,单个细胞,少数聚团 |
| 细胞培养条件 | RPMI-1640+10% FBS+1% P/S |
| 培养条件气体 | 空气,95%;CO2,5%;温度:37℃ |
| 推荐冻存条件 | 30% 基础培养基+60%FBS+10%DMSO |
| 冻存液氮 | 液氮保存 |
| 推荐传代比例 | 1:3--1:4 |
| 推荐换液频率 | 2-3次/周 |
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