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pCMV-BKCA-FLAG人源基因哺乳表达质粒

  • 货号：KL-ZL1833-5ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL1833-5ug pCMV-BKCA-FLAG人源基因哺乳表达质粒 5ug

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》产品信息

货号 名称 规格
KL-ZL1833 pCMV-BKCA-FLAG人源基因哺乳表达质粒

5ug

》产品描述

别名： NM_006516.2 原核抗性： 氨苄青霉素Amp 筛选标记： 遗传霉素G418 克隆菌株： 大肠杆菌DH5α 培养条件： 37℃

》产品简介

pECMV-3×FLAG-SLC2A1是一个人源基因哺乳表达质粒。

》产品图谱及序列


序列：
CDS complement(5941..6801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6802..6906)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac
961 atcgactaca aggatgacga tgacaagctt ggtaccgagc tcggatccgc caccatggag
1021 cccagcagca agaagctgac gggtcgcctc atgctggccg tgggaggagc agtgcttggc
1081 tccctgcagt ttggctacaa cactggagtc atcaatgccc cccagaaggt gatcgaggag
1141 ttctacaacc agacatgggt ccaccgctat ggggagagca tcctgcccac cacgctcacc
1201 acgctctggt ccctctcagt ggccatcttt tctgttgggg gcatgattgg ctccttctct
1261 gtgggccttt tcgttaaccg ctttggccgg cggaattcaa tgctgatgat gaacctgctg
1321 gccttcgtgt ccgccgtgct catgggcttc tcgaaactgg gcaagtcctt tgagatgctg
1381 atcctgggcc gcttcatcat cggtgtgtac tgcggcctga ccacaggctt cgtgcccatg
1441 tatgtgggtg aagtgtcacc cacagccctt cgtggggccc tgggcaccct gcaccagctg
1501 ggcatcgtcg tcggcatcct catcgcccag gtgttcggcc tggactccat catgggcaac
1561 aaggacctgt ggcccctgct gctgagcatc atcttcatcc cggccctgct gcagtgcatc
1621 gtgctgccct tctgccccga gagtccccgc ttcctgctca tcaaccgcaa cgaggagaac
1681 cgggccaaga gtgtgctaaa gaagctgcgc gggacagctg acgtgaccca tgacctgcag
1741 gagatgaagg aagagagtcg gcagatgatg cgggagaaga aggtcaccat cctggagctg
1801 ttccgctccc ccgcctaccg ccagcccatc ctcatcgctg tggtgctgca gctgtcccag
1861 cagctgtctg gcatcaacgc tgtcttctat tactccacga gcatcttcga gaaggcgggg
1921 gtgcagcagc ctgtgtatgc caccattggc tccggtatcg tcaacacggc cttcactgtc
1981 gtgtcgctgt ttgtggtgga gcgagcaggc cggcggaccc tgcacctcat aggcctcgct
2041 ggcatggcgg gttgtgccat actcatgacc atcgcgctag cactgctgga gcagctaccc
2101 tggatgtcct atctgagcat cgtggccatc tttggctttg tggccttctt tgaagtgggt
2161 cctggcccca tcccatggtt catcgtggct gaactcttca gccagggtcc acgtccagct
2221 gccattgccg ttgcaggctt ctccaactgg acctcaaatt tcattgtggg catgtgcttc
2281 cagtatgtgg agcaactgtg tggtccctac gtcttcatca tcttcactgt gctcctggtt
2341 ctgttcttca tcttcaccta cttcaaagtt cctgagacta aaggccggac cttcgatgag
2401 atcgcttccg gcttccggca ggggggagcc agccaaagtg acaagacacc cgaggagctg
2461 ttccatcccc tgggggctga ttcccaagtg tgactcgagt ctagagggcc cgtttaaacc
2521 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
2581 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
2641 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
2701 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
2761 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
2821 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
2881 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
2941 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
3001 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
3061 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
3121 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
3181 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
3241 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
3301 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
3361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
3421 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
3481 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
3541 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
3601 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
3661 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
3721 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
3781 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
3841 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
3901 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
3961 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
4021 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
4081 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
4141 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
4201 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
4261 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
4321 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
4381 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
4441 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
4501 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
4561 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
4621 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
4681 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
4741 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
4801 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
4861 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
4921 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
4981 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
5041 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
5101 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
5161 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
5221 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
5281 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
5341 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
5401 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
5461 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
5521 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
5581 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
5641 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
5701 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
5761 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
5821 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
5881 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
5941 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
6001 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
6061 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
6121 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
6181 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
6241 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
6301 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
6361 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
6421 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
6481 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
6541 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
6601 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
6661 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
6721 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
6781 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
6841 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
6901 tccgcgcaca tttccccgaa aagtgccacc tgacgtc

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