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YEplac195酿酒酵母表达质粒

货号：KL-ZL1807-5ug

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL1807-5ug	YEplac195酿酒酵母表达质粒	5ug		立即咨询	+ -

产品介绍
相关产品

》产品信息

货号	名称	规格
KL-ZL1807	YEplac195酿酒酵母表达质粒	5ug

》产品描述

原核抗性：氨苄青霉素Amp 筛选标记： URA3 克隆菌株：大肠杆菌DH5α 培养条件： 37℃

》产品简介

YEplac195是一个酿酒酵母表达质粒。

》产品图谱及序列

序列：

CDS 3439..4299

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

rep_origin 4470..5058

/direction=RIGHT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

ORIGIN

1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca

61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct

121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat

181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc

241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg

301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac

361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac

421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt

481 gcggtatttc acaccgcata atcggatcgt acttgttacc catcattgaa ttttgaacat

541 ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata ataatatata

601 gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac tattgcatct

661 attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca

721 cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga acaaaaatgc

781 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa

841 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa

901 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac

961 agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct

1021 acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc

1081 tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag

1141 gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact

1201 tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc

1261 ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt

1321 tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac

1381 tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt

1441 actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg

1501 agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca

1561 cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata

1621 ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc

1681 gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagctagag aataggaact

1741 tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc

1801 tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata

1861 tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct

1921 atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg

1981 gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat

2041 tggattagtc tcatccttca atgctatcat ttcctttgat attggatcga tccgatgata

2101 agctgtcaaa catgagaatt gggtaataac tgatataatt aaattgaagc tctaatttgt

2161 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt

2221 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt

2281 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca

2341 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt

2401 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag

2461 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca

2521 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt

2581 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt

2641 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg

2701 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg

2761 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca

2821 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg

2881 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat

2941 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac

3001 atgatttatc ttcgtttcct gcatgttttt gttctgtgca gttgggttaa gaatactggg

3061 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc

3121 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aagaaaccga

3181 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt gaaaagctaa ttcttgaaga

3241 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct

3301 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc

3361 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa

3421 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt

3481 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct

3541 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc

3601 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta

3661 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac

3721 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc

3781 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac

3841 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg

3901 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac

3961 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc

4021 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt

4081 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga

4141 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc

4201 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag

4261 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca

4321 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc

4381 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca

4441 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc

4501 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta

4561 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt

4621 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc

4681 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg

4741 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg

4801 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag

4861 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc

4921 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat

4981 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg

5041 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc

5101 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt

5161 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca

5221 gtgagcgagg aagcggaaga

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