别名： NM_013240.5
启动子： CMV
复制子： pUC
终止子： WPRE
原核抗性： 氨苄青霉素Amp
筛选标记： 嘌呤霉素Puro
克隆菌株： 大肠杆菌Stbl3
培养条件： 37℃
pLVML-3×FLAG-N6AMT1-IRES-Puro(NM_013240.5)是一个人源基因慢病毒哺乳细胞过表达质粒。
序列：
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggac
2821 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
2881 aagctcgaga tggcagggga gaacttcgct acgccgttcc acgggcacgt gggccgcggc
2941 gccttcagcg acgtgtacga gcccgcggag gacacgtttc tgcttttgga cgcgctggag
3001 gcagcggctg ccgaactggc aggagtggaa atatgcctgg aagtagggtc agggtctggt
3061 gtagtatctg cattcctagc ctctatgata ggccctcagg ctttgtacat gtgcactgat
3121 atcaaccctg aggcagcagc ttgtacccta gagacagcac gctgtaacaa agttcacatt
3181 caaccagtta ttacagattt ggtcaaaggc ttgctaccaa gattgaccga aaaagttgat
3241 cttctggtgt ttaatccccc ctatgtagtg actccacctc aagaggtagg aagtcacgga
3301 atagaggcag cttgggctgg tggcagaaat ggtcgggaag tcatggacag gttttttccc
3361 ctggttccag atctcctttc accaagagga ttattctatt tagttaccat taaagaaaac
3421 aacccagaag aaattttgaa aataatgaag acaaaaggtc tgcaaggaac cactgcactt
3481 tccagacaag caggccaaga aactctttca gtcctcaagt tcaccaagtc ttagggatcc
3541 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
3601 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
3661 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
3721 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
3781 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
3841 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
3901 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
3961 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
4021 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
4081 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc acaaggagac
4141 gaccttccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
4201 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
4261 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
4321 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
4381 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
4441 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
4501 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
4561 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
4621 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
4681 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
4741 gtgcctagac gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
4801 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
4861 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
4921 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
4981 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
5041 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
5101 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
5161 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
5221 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
5281 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
5341 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
5401 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
5461 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
5521 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
5581 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
5641 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
5701 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
5761 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
5821 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
5881 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
5941 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
6001 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
6061 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
6121 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
6181 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
6241 tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac ctctagctag
6301 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
6361 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
6421 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
6481 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
6541 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
6601 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
6661 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
6721 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
6781 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
6841 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
6901 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
6961 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
7021 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
7081 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
7141 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
7201 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
7261 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
7321 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
7381 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
7441 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
7501 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
7561 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
7621 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
7681 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
7741 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
7801 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
7861 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
7921 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
7981 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
8041 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
8101 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
8161 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
8221 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
8281 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
8341 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
8401 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
8461 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta
8521 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
8581 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
8641 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
8701 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
8761 tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata
8821 tgtactacaa acttagtagt
