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pGM1190链霉菌表达质粒

货号：KL-ZL1615-2ug

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL1615-2ug	pGM1190链霉菌表达质粒	2ug		立即咨询	+ -

产品介绍
相关产品

》产品信息

货号	名称	规格
KL-ZL1615	pGM1190链霉菌表达质粒	2ug

》产品描述

启动子： TipA promoter 质粒大小： 7089bp 质粒标签： N-6xHis/thrombin site/T7 tag，C-6xHis 原核抗性：安普霉素Apr 克隆菌株：大肠杆菌DH5α 培养条件：有氧，LB,37℃ 表达宿主：链霉菌

》产品简介

pGM1190是一个链霉菌表达载体，通过TipA 启动子驱动目的基因的表达。转化进链霉素的时候请用35℃的温度培养。OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces，replicates also in Streptomyces (below 35°C).

》产品图谱及序列

序列：

ORIGIN

1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg

61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc

121 cgaagcggta cgcgaacccg tgccgggctg cggcggatcg cggcagcagg agctccccgc

181 ccgccacggc tggctggatg gtcgcctcac gcgcccgcag gggtgacggc gtgggtgcgg

241 cggtcagccc cgtgatcggg cgtcagacgg ccgctgaggg accgcgacca cccctccctt

301 cccgcctgcc gtctttcacc gcccggccgg tccgcagacg ccgtacggcc aggccacgga

361 ggattacggg cagatggcgc ccacccggca cccgggctgg ggcaccctgg acagcacgaa

421 ggcccgcccc actcaccggg gcgggccttc gcgttgagct agagttcccg ccagcctcgc

481 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac

541 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa

601 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat

661 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa tgcagctcac

721 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa

781 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt

841 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca

901 gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt

961 ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc

1021 cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctgc gctgggtccg

1081 ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc

1141 acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat

1201 gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc

1261 atctctgatc cattgcccct gccacctcac tcgcctgcaa gcccggtcgc ccgtgtccat

1321 gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat

1381 cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag

1441 gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc

1501 ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc

1561 tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga

1621 gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc

1681 cagtcgattg gctgagctca taagttccta ttccgaagtt cctattcgaa atgaccgacc

1741 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt

1801 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca

1861 tgctggagtt cttcgcccac cccaaaagga tctaggtgaa gatccttttt gataatctca

1921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga

1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa

2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga

2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt

2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt

2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat

2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct

2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca

2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag

2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc

2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga

2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca

2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag

2701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg

2761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct

2821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt

2881 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg

2941 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattgt

3001 acgtaggcgt cagctacgat gttccgggga ctgctgatcc ggtcagcagg tggaagaggg

3061 actggattcc aaagttctca atgctgcttg ctgttcttga atggggggtc gttgacgacg

3121 acatggctcg attggcgcga caagttgctg cgattctcac caataaaaaa cgcccggcgg

3181 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg accggatcgg

3241 ggatctgggc tgagggagcc gacggcacgc ggcggctcac ggcgtggcac gcggaacgtc

3301 cgggcttgca cctcacgtca cgtgaggagg cagcgtggac ggcgtcagag aagggagcgg

3361 accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc

3421 catatggcta gcatgactgg tggacagcaa atgggtcgcg gatccgaatt cgagctccgt

3481 cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa

3541 cccagatcta aagttttgtc gtctttccag acgttagtaa atgaattttc tgtatgaggt

3601 tttgctaaac aactttcaac agtttcagcg gagtgagaat agaaaggaac aactaaagga

3661 attgcgaata ataatttttt cacgttgaaa atctccaaaa aaaaaggctc caaaaggagc

3721 ctttaattgt atcggtttat cagcttgctt tcgaggtgaa tttcttaaac agcttgatac

3781 cgatagttgc gccgacaatg acaacaacca tcgcccacgc ataaccgata tattcggtcg

3841 ctgaggcttg cagggagtca aaggccgctt ttgcgggatc tcgtcgaagg cactagaggc

3901 ctatatgcgg ggatcgaccg cgcgggtccc ggacggggaa gagcggggag ctttgccaga

3961 gagcgacgac ttccccttgc gttggtgatt gccggtcagg gcagccatcc gccatcgtcg

4021 cgtagggtgt cacaccccag gaatcgcgtc actgaacaca gcagccggta ggacgaccat

4081 gactgagttg gacaccatcg caaatccgtc cgatcccgcg gtgcagcgga tcatcgatgt

4141 caccaagccg tcgcgatcca acataaagac aacgttgatc gaggacgtcg agcccctcat

4201 gcacagcatc gcggccgggg tggagttcat cgaggtctac ggcagcgaca gcagtccttt

4261 tccatctgag ttgctggatc tgtgcgggcg gcagaacata ccggtccgcc tcatcgactc

4321 ctcgatcgtc aaccagttgt tcaaggggga gcggaaggcc aagacattcg gcatcgcccg

4381 cgtccctcgc ccggccaggt tcggcgatat cgcgagccgg cgtggggacg tcgtcgttct

4441 cgacggggtg aagatcgtcg ggaacatcgg cgcgatagta cgcacgtcgc tcgcgctcgg

4501 agcgtcgggg atcatcctgg tcgacagtga catcaccagc atcgcggacc ggcgtctcca

4561 aagggccagc cgaggttacg tcttctccct tcccgtcgtt ctctccggtc gcgaggaggc

4621 catcgccttc attcgggaca gcggtatgca gctgatgacg ctcaaggcgg atggcgacat

4681 ttccgtgaag gaactcgggg acaatccgga tcggctggcc ttgctgttcg gcagcgaaaa

4741 gggtgggcct tccgacctgt tcgaggaggc gtcttccgcc tcggtttcca tccccatgat

4801 gagccagacc gagtctctca acgtttccgt ttccctcgga atcgcgctgc acgagaggat

4861 cgacaggaat ctcgcggcca accgataagc gcctctgttc ctcggacgct cggttgctcg

4921 acctcgattc gtcagtgatg atcaacagga cccagaaatg gcacgagccc gggattggcc

4981 tcccgggctc gccgcccccg aaccggcgca gcatgccgag tgaggacgtt tgctctgtga

5041 cgagcgtacg agagaacagc gcgtcgacac caccgcgccc cgccggaccg aagtgccact

5101 gcggcacccc gatcgtccac atccccggga agcgccccaa aatctactgc tcggacgcct

5161 gccgaaagcg cgcaaagcgt ctcattgcgg accagtcacg cagcgtagca agcacgacta

5221 cagcgggtaa tccggacgcg ggctttagta gtaagggaaa atctccgtcc actgcaggtc

5281 agagcgctgt aactggaacg ggttctaccc caaacgaggg tgccgcgaca gccgggcggg

5341 aggacccgcg cgacgagcgg ttcgcccggc gggaccggca ccagacggtg tcgctgaacg

5401 aggcgttcaa gggctgcggg acccggctca ctgccgggac ggccgagctg atgtggaagc

5461 cgggcgaggc gacgtggggc aacacgtgcc gctgcaacaa cgtccacacc tgcccgtggt

5521 gcatgagccg catcctggcc gtacggggct cgaacgtgca gctcgccgcc gacggtctcg

5581 cggacgccgg gtacgggctg caccttggca cgaacacgtt gcgccacttc gagcgcatgg

5641 cgttcggcac cgtccgcaag ggaatgcggc acggcctggt cgcggtcttg cacgacggct

5701 ggaagggcgc gtacggctcg tccggccgcc gctggcgcac gatgcgcgac gacttcggga

5761 tcatcggcta cgagcgggcc ttcgaggaca ccttcggctg gggctccggc tggcacctgc

5821 actggcacac gctctgggtc acccgcgagg tgctcggccc ggacgcccag gccgcgttcc

5881 gcgacgcact cgccggagcc tgggccgccg gggtcgaatc cgcgggcggc tacaccgtca

5941 gcgagacgtg cgaccggccc ggttgctcct gtgagggcaa gggccacggc accgacgtgc

6001 gcccactcaa cggcgcggac gccgctgacg gcgatgcggg caagcaggcc cgctacctct

6061 acaaggacgg cgacaagacc aagggcggcg tcgccaagat cggcctggag ctggccggac

6121 agaacttcaa ggccggtcgc ggcgacgacc gcatgggacc gctcgacctg ggcgacgcgg

6181 cggccgccga gctgcagcgg cttcgtcggc ccggcccgtt cgtcgagaag taccgcgagc

6241 gcgagttcgg cgtcttccag gtccgcaagc actaccggtc gcagaacctg aaccggctga

6301 tcaaggaact cggcatccag caggacgtcc gcaccgagga ggagatcacc gacgacaccg

6361 agggcctggt cgccatcgcg gtcatcccgg cctacatctg gtaccgatac atcgcccgcg

6421 tcgccggacg tcgcctcgac ctcatcaagg tcgccgagac gtacggccta ccgggcgtcc

6481 ggcggctcgt cgagtcctgg ggcctggtgt ggggcaagga cgtcctggac ccgcccgccc

6541 cggaagcgcc ggccgcgccg ggcgaccttg acgctgacca gatgaggttc gaggtcatgt

6601 ccgaggagga agctgctttc cgcgaggcgc gccggaaggc caacgaggcc cgcacggagg

6661 agctggccgc ttcgctcgac cgagtgcgtc agccgaagaa ggaggcgatc cgcccgacga

6721 tcagccttcg gaagcgcctg aagccgaagc cggtcaccgt cgacgtcaag actcccccgc

6781 ccggcgccgc gtcgcccgtg tgccgccgct gcaagggcaa gctcgccccg gtactccagc

6841 cctggggccg gctccccgga gactgcctcc gcgtcgacac agcggtcgcg tagggccgct

6901 gagggaccgc catgagaccg ccaccccgga catccgggtg gcggtctcat ggcggtcccg

6961 gcgcgaaagc cgagggtctg cctgccgtga ggtggccggc ggacctgggt tactgctgga

7021 atcccgcgcc cagtccgagc gtccgccggc gcttttcacc tggctccggg tgctcgtcga

7081 tgagcacca

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