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pPSU2DNA Marker制备质粒

  • 货号：KL-ZL1604-2ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL1604-2ug pPSU2DNA Marker制备质粒 2ug

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》产品信息

货号 名称 规格
KL-ZL1604 pPSU2DNA Marker制备质粒

2ug

》产品描述

复制子： pUC ori 质粒大小： 7750bp 原核抗性： 氨苄青霉素Amp 克隆菌株： 大肠杆菌DH5α 培养条件： 37℃，有氧，LB 备注： 高拷贝

》产品简介

pPSU2质粒是一个用于制备DNA Marker的高拷贝质粒，如果用Pst1单酶切，会出现50bp,100bp,200bp,300bp,400bp,500bp,600bp,1500bp,4000bp等条带，如果用EcoRI单酶切，会出现750bp,3000bp,4000bp等条带。

》产品图谱及序列


序列：
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttga
241 tatcatcaaa gccatgaaca aagcagccgc gctggatgaa ctgataccgg ggttgctgag
301 tgaatatatc gaacagtcag gttaacctgc agcggcattt tgtccgcgcc gggcttcgct
361 cactgttcag gccggagcca cagaccgccg ttgaatgggc ggatgctaat tactatctcc
421 cgaaagaatc cgcataccag gaagggcgct gggaaacact gccctttcag cgggccatca
481 tgaatgcgat gggcagcgac tacatccgtg aggtgaatgt ggtgaagtct gcccgtgtcg
541 gttattccaa aatgctgctg ggtgtttatg cctactttat agagcataag cagcgcaaca
601 cccttatctg gttgccaacg gatggtgatg ccgagaactt tatgaaaacc cacgttgagc
661 cgactattcg tgatattccg tcgctgctgg cgctggcccc gtggtatggc aaaaagcacc
721 gggataacac gctcaccatg aagcgtttca ctaatgggcg tggcttctgg tgcctgggcg
781 gtaaagcggc aaaaaactac cgtgaaaagt cggtggatgt ggcgggttat gatgaacttg
841 ctgcttttga tgatgatatt gaacaggaag gctctccgac gttcctgggt gacaagcgta
901 ttgaaggctc ggtctggcca aagtccatcc gtggctccac gccaaaagtg agaggcacct
961 gtcagattga gcgtgcagcc agtgaagcga tatcggatct ttccgagcat ttattaagca
1021 tttcgctata agttctcgct ggaagaggta gttttttcat tgtactttac cttcatctct
1081 gttcattatc atcgctttta aaacggttcg accttctaat cctatctgac cattataatt
1141 ttttagaatg gtttcataag aaagctctga atcaacggac tgcgataata agtggtggta
1201 tccagaattt gtcacttcaa gtaaaaacac ctcacgagtt aaaacaccta agttctcacc
1261 gaatgtctca atatccggac ggataatatt tattgcttct cttgaccgta ggactttcca
1321 catgcaggat tttggaacct cttgcagtac tactggggaa tgagttgcaa ttattgctac
1381 accattgcgt gcatcgagta agtcgcttaa tgttcgtaaa aaagcagaga gcaaaggtgg
1441 atgcagatga acctctggtt catcgaataa aactaatgac ttttcgccaa cgacatctac
1501 taatcttgtg atagtaaata aaacaattgc atgtccagag ctcattcgaa gcagatattt
1561 ctggatattg tcataaaaca atttagtgaa tttatcatcg tccacttgaa tctgtggttc
1621 attacgtctt aactcttcat atttagaaat gaggctgatg agttccatat ttgaaaagtt
1681 ttcatcacta cttagttttt tgatagcttc aagccagagt tgtctttttc tatctactct
1741 catacaacca ataaatgctg aaatgaattc taagcggaga tcgcctagtg attttaaact
1801 attgctggca gcattcttga gtccaactgc agagtattgt gtaccttttg ctgggtcagg
1861 ttgttcttta gccggcctgc aggaagagga tggtgcagca accaacaaga aaacactggc
1921 agattacgcc cgtgccttat ccggagagga tgaatgacgc gacaggaaga acttgcctgc
1981 agtaactttc cccacaacgg aacaactctc attgcatggg atcattgggt actgtgggtt
2041 tagtggttgt aaaaacacct gaccgctatc cctgatcagt ttcttgaagg taaactcatc
2101 acccccaagt ctggctatgc agaaatcacc tggctcaaca gcctgctcag ggtcaacgag
2161 aattaacatt ccgtcactgc agacggctca ggatacggat aacggctact ccgtgtttga
2221 gcagtcactg ctgcggtata tcgctgccgg gctgggtgtc tcgtatgagc agctttcccg
2281 gaattacgcc cagatgagct actccacggc acgggccagt gcgaacgagt cgtgggcgta
2341 ctttatgggg cggcgaaaat tcgtcgcatc ccgtcaggcg agccagatgt ttctgtgctg
2401 gctggaagag gccatcgttc gccgcgtggt gacgttacct tcaaaagcgc gcttcagttt
2461 tcaggaagcc agatctctgc aggttgacgg ttttccacca tcgcacgccg ggaccatcac
2521 cgtgtatgaa gattcacaac cggggacgct gaatgatttt ctctgtgcca tgacggagga
2581 tgatgcccgg ccggaggtgc tgcgtcgtct tgaactgatg gtggaagagg tggcgcgtaa
2641 cgcgtccgtg gtggcacaga gtacggcaga cgcgaagaaa tcagccggcg atgccagtgc
2701 atcagctgct caggtcgcgg cccttgtgac tgatgcaact gactcagcac gcgccgccag
2761 cacgtccgcc ggacaggctg catcgtcagc tcaggaagcg tcctccggcg cagaagcggc
2821 atcagcaaag gccactgaag cggaaaaaag tgccgcagcc gcagagtcct caaaaactgc
2881 aggcggatta caacacgctg atggcggcgg cgaaaaagga ttatgaagcg acgctgaaaa
2941 agccgaaaca gtccagcgtg aaggtgtctg cgggcgatcg tcaggaagac agtgctcatg
3001 ctgccctgct gacgcttcag gcagaactcc ggacgctgga gaagcatgtc ggagcaaatg
3061 agaaaatcag ccagcagcgc cgggatttgt ggaaggcgga gagtcagttc gcggtactgg
3121 aggaggcggc gcaacgtcgc cagctgtctg cacaggagaa atccctgctg gcgcataaag
3181 atgagacgct ggagtacaaa cgccagctgg ctgcacttgg cgacaaggtt acgtatcagg
3241 agcgcctgaa cgcgctggcg cagcaggcgg ataaattcgc acagcagcaa cgggcaaaac
3301 gggccgccat tgatgcgaaa agccgggggc tgactgaccg gcaggcagaa cgggaagcca
3361 cggaacagcg cctgaactgc agggatccct tctggggccg gacggcatga catcgctgcg
3421 cgaatatgcc ggttatcacg gcggtggcag cggatttgga gggcagttgc ggtcgtggaa
3481 cccaccgagt gaaagtgtgg atgcagccct gttgcccaac tttacccgtg gcaatgcccg
3541 cgcagacgat ctggtacgca ataacggcta tgccgccaac gccatccagc tgcatcagga
3601 tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat
3661 cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc
3721 cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg
3781 ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac
3841 cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa
3901 cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg
3961 tgcggcgctg ggacatctgc agtgtacaga tatccaaatg aagccatagg catttgttat
4021 tttggctctg tcagctgcat aacgccaaaa aatatattta tctgcttgat cttcaaatgt
4081 tgtattgatt aaatcaattg gatggaattg tttatcataa aaaattaatg tttgaatgtg
4141 ataaccgtcc tttaaaaaag tcgtttctgc aagcttggct gtatagtcaa ctaactcttc
4201 tgtcgaagtg atatttttag gcttatctac cagttttaga cgctctttaa tatcttcagg
4261 aattatttta ttgtcatatt gtatcatgct aaatgacaat ttgcttatgg agtaatcttt
4321 taattttaaa taagttattc tcctggcttc atcaaataaa gagtcgaatg atgttggcga
4381 aatcacatcg tcacccattg gattgtttat ttgtatgcca agagagttac agcagttata
4441 cattctgcca tagattatag ctaaggcatg taataattcg taatctttta gcgtattagc
4501 gacccatcgt ctttctgatt taataataga tgattcagtt aaatatgaag gtaatttctt
4561 ttgtgcaagt ctgactaact tttttatacc aatgtttaac atactttcat ttgtaataaa
4621 ctcaatgtca ttttcttcaa tgtaagatga aataagagta gcctttgcct cgctatacat
4681 ttctaaatcg ccttgttttt ctatcgtatt gcgagaattt ttagcccaag ccattaatgg
4741 atcatttttc catttttcaa taacattatt gttataccaa atgtcatatc ctataatctg
4801 gtttttgttt ttttgaataa taaatgttac tgttcttgcg gtttggagga attgattcaa
4861 attcaagcga aataattcag ggtcaaaata tgtatcaatg cagcatttga gcaagtgcga
4921 taaatcttta agtcttcttt cccatggttt tttagtcata aaactctcca ttttgatagg
4981 ttgcatgcta gatgctgata tattttagag gtgataaaat taactgctta actgtcaatg
5041 taatacaagt tgtttgatct ttgcaatgat tcttatcaga aaccatatag taaattagtt
5101 acacaggaaa tttttaatat tattattatc attcattatg tattaaaatt agagttgtgg
5161 cttggctctg ctaacacgtt gctcatagga gatatggtag agccgcagac acgtcgtatg
5221 caggaacgtg ctgtggctgg ctggtgaact tccgatagtg cgggtgttga atgatttcca
5281 gttgctaccg attttacata ttttttgcat gagagaattt gtaccacctc ccaccgacca
5341 tctatgagaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
5401 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
5461 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
5521 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
5581 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
5641 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
5701 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
5761 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
5821 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
5881 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
5941 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
6001 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
6061 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
6121 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
6181 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
6241 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
6301 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
6361 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
6421 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
6481 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
6541 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
6601 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
6661 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
6721 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
6781 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
6841 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
6901 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
6961 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
7021 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
7081 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
7141 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
7201 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
7261 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
7321 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
7381 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
7441 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
7501 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
7561 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
7621 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
7681 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
7741 aagcggaaga

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