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pCMV-ABE7.9质粒

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL-1216-01	pCMV-ABE7.9质粒	NA		立即咨询	+ -

哺乳基因编辑质粒货号：KL-ZL-1216 规格：5ug

BACKBONE

Vector backbone pBR322/CMV

(Search Vector Database)

Backbone manufacturer Liu lab

Backbone size w/o insert (bp) 3388

Total vector size (bp) 8727

Vector type Mammalian Expression

GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin

Growth Temperature 37°C

Growth Strain(s) DH5alpha

Copy number High Copy

GENE/INSERT

Gene/Insert name ABE7.9

Alt name TadA-linker(32aa)-TadA*(7.9)-linker(32aa)-nCas9-NLS

Species Synthetic; TadA is from E.coli (synthetic gene block, mammalian codon optimized), Cas9 is S. pyogenes (mammalian codon optimized)

Insert Size (bp) 5328

Mutation see manuscript

Promoter CMV

Tag / Fusion Protein

NLS (C terminal on insert)

CLONING INFORMATION

Cloning method Ligation Independent Cloning

5′ sequencing primer T7

3′ sequencing primer M13

(Common Sequencing Primers)