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pSecTag2 A质粒

货号：kl-zl-1071-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1071-01	pSecTag2 A质粒	NA		立即咨询	+ -

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pSecTag2 A质粒货号：kl-zl-1071 规格：20ul

启动子：	CMV,SV40,T7
复制子：	pUC ori，FI ori
终止子：	SV40 poly(A) signal,BGH poly(A) signal
质粒大小：	5159bp
原核抗性：	Amp
筛选标记：	Neo
克隆菌株：	DH5α
培养条件：	37℃ 有氧，LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	T7：TAATACGACTCACTATAGGG
3'测序引物：	BGH：TAGAAGGCACAGTCGAGG

质粒简介：
pSecTag2 A, B, and C vectors are fusion vectors requiring that you clone your gene of interest in frame with the initiation ATG of the N-terminal Igκ-chain leader sequence and/or the C-terminal myc epitope/polyhistidine tag. Three versions of this vector are provided to facilitate cloning. For proper expression,first determine which restriction sites are appropriate for ligation and then which vector will preserve the reading frame at BOTH the 5′ and the 3′ ends. It may be nece ssary to PCR your gene product to create a fragment with the appropriate restriction sites to clone in frame at both ends. Carefully inspect your gene and the multiple cloning site of each vector before cloning your gene of interest
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