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pEBFP-N1质粒

货号：kl-zl-1059-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1059-01	pEBFP-N1质粒	NA		立即咨询	+ -

产品介绍
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pEBFP-N1质粒货号：kl-zl-1059 规格：20ul

启动子：	CMV
复制子：	pUC ori,f1 ori
终止子：	SV40 poly(A) signal
质粒分类：	哺乳细胞，荧光蛋白报告载体
质粒大小：	4733bp
原核抗性：	Kan
筛选标记：	Neo
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物：	根据序列设计引物

质粒简介：
pEBFP-N1 carries a blue fluorescent variant of the Aequorea victoria green fluorescent protein gene (GFP). The EBFP gene contains four amino acid substitutions. The Tyr-66 to His substitution gives EBFP fluorescence excitation and emission maxima (380 and 440 nm, respectively) similar to other blue emission variants (1–3). The other three substitutions (Phe-64 to Leu; Ser-65 to Thr; and Tyr- 145 to Phe) enhance the brightness and solubility of the protein, primarily due to improved proteinfolding properties and efficiency of chromophore formation (1, 4, 5). The Em of EBFP is 31,000 cm–1M–1 for 380-nm excitation, leading to a fluorescent signal that is 2–3-fold brighter than other blue variants of GFP and roughly equivalent to wt GFP. In addition, the rate of photobleaching of EBFP is one-half to one-third that of P4-3, a popular predecessor to EBFP (1). EBFP contains >190 silent mutations that create an open reading frame comprised almost entirely of preferred human codons (6). Furthermore, upstream sequences flanking EBFP have been converted to a Kozak consensus translation initiation site (7). These changes increase the translational efficiency of the EBFP mRNA and consequently the expression of EBFP in mammalian and plant cells.
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