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pGL4.19质粒

货号：kl-zl-1027-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1027-01	pGL4.19质粒	NA		立即咨询	+ -

产品介绍
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pGL4.19质粒货号：kl-zl-1027 规格：20ul

启动子：	SV40
复制子：	pUC ori，SV40 ori
终止子：	SV40 poly(A) signal
质粒分类：	哺乳细胞，信号通路报告载体
质粒大小：	5778bp
原核抗性：	Amp
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	Sv40-polyA-R：GAAATTTGTGATGCTATTGC
3'测序引物：	根据序列设计引物

质粒简介：
The pGL4.19[luc2CP/Neo] Vector encodes the luciferase reporter gene luc2CP (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. This vector is engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression. The pGL4.19[luc2CP/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The luc2CP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by luc2CP responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.
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