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pAAV-IRES-hrGFP质粒

  • 货号:kl-zl-0984-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0984-01 pAAV-IRES-hrGFP质粒 NA

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pAAV-IRES-hrGFP质粒 货号:kl-zl-0984 规格:20ul

启动子: CMV,T7
复制子: pUC ori,f1 ori
终止子: hGH poly(A) signal
质粒分类: 病毒系列,腺病毒相关克隆载体
质粒大小: 6.1kb
原核抗性: Amp
克隆菌株: Stbl3
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: T7:TAATACGACTCACTATAGGG
3'测序引物: hGH-PA-R: CCAGCTTGGTTCCCAATAGA
质粒简介:
The vector pAAV-IRES-hrGFP contains a dicistronic expression cassette in which the Renilla reniformis green fluorescent protein (hrGFP) gene is expressed as a second open reading frame downstream of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES). The hrGFP gene serves as a useful marker for titering the virus and ascertaining the infection efficiency for the desired target cell type. Another unique feature of this vector is the FLAG sequence (3x) at the c-terminal of MCS. The foreign gene could be cloned in frame with the tag and the protein could be further purified or detected by anti-FLAG antibody.
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