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pPINK-LC质粒

货号：kl-zl-0891-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0891-01	pPINK-LC质粒	NA		立即咨询	+ -

产品介绍
相关产品

pPINK-LC质粒货号：kl-zl-0891 规格：20ul

启动子：	PpADE2 LC，AOX1
复制子：	pUC ori
终止子：	CYC1
质粒分类：	酵母系列，毕赤酵母表达载体
质粒大小：	7732bp
原核抗性：	Amp
筛选标记：	ADE2
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	酵母细胞
5'测序引物：	AOX5：GACTGGTTCCAATTGACAAGC
3'测序引物：	CYC1-R： GCGTGAATGTAAGCGTGAC

质粒简介：
he PichiaPink™ Vector Kit contains the pPINK-HC and pPINK-LC vectors for use with the PichiaPink™ Yeast Expression System. The pPINK vectors are built around the ADE2 gene for complementing the ade2 deletion in the PichiaPink™ strains. You can order the PichiaPink™ Vector Kit to refill your PichiaPink™ Yeast Expression System Kits.
质粒图谱：

序列：

primer_bind 7710..7726

/note="M13 fwd"

/note="common sequencing primer, one of multiple similar

variants"

ORIGIN

1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag

61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt

121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc

181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta

241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta

301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg

361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct

421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg

481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt

541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct

601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct

661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact

721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat

781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt

841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga

901 caacttgaga agatcaaaaa acaactaatt attcgaaacg gaattccgga ccggcatgca

961 ggcctggtac cggccggcca tttaaataca ggcccctttt cctttgtcga tatcatgtaa

1021 ttagttatgt cacgcttaca ttcacgccct cctcccacat ccgctctaac cgaaaaggaa

1081 ggagttagac aacctgaagt ctaggtccct atttattttt tttaatagtt atgttagtat

1141 taagaacgtt atttatattt caaatttttc ttttttttct gtacaaacgc gtgtacgcat

1201 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt

1261 gcaagctgga tccgcggccg cgacttgact cttcactagc ctatgcaaat aaggttacct

1321 tttccaagaa tcgtagaaac gattaaaaaa cttccaaact ctcatggatt ctcaggtaat

1381 aggtattcta ggaggaggcc agctaggccg aatgattgtt gaggccgcta gcaggctcaa

1441 tatcaagacc gtgattcttg atgatggttt ttcacctgct aagcacatta atgctgcgca

1501 agaccacatc gacggatcat tcaaagatga ggaggctatc gccaagttag ctgccaaatg

1561 tgatgttctc actgtagaga ttgagcatgt caacacagat gctctaaaga gagttcaaga

1621 cagaactgga atcaagatat atcctttacc agagacaatc gaactaatca aggataagta

1681 cttgcaaaag gaacatttga tcaagcacaa catttcggtg acaaagtctc agggtataga

1741 atctaatgaa aaggcgctgc ttttgtttgg agaagagaat ggatttccat atctgttgaa

1801 gtcccggact atggcttatg atggaagagg caattttgta gtggagtcta aagaggacat

1861 cagtaaggca ttagagttct tgaaagatcg tccattgtat gccgagaagt ttgctccttt

1921 tgttaaagaa ttagcggtaa tggttgtgag atcactggaa ggcgaagtat tctcctaccc

1981 aaccgtagaa actgtgcaca aggacaatat ctgtcatatt gtgtatgctc cggccagagt

2041 taatgacacc atccaaaaga aagctcaaat attagctgaa aacactgtga agactttccc

2101 aggcgctgga atcttcggag ttgagatgtt cctattgtct gatggagaac ttcttgtaaa

2161 tgagattgct ccaaggcccc acaattctgg tcactataca atcgatgcat gtgtaacatc

2221 tcagttcgaa gcacatgtaa gagccataac tggtctgcca atgccactag atttcaccaa

2281 actatctact tccaacacca acgctattat gctcaatgtt ttgggtgctg aaaaatctca

2341 cggggaatta gagttttgta gaagagcctt agaaacaccc ggtgcttctg tatatctgta

2401 cggaaagacc acccgattgg ctcgtaagat gggtcatatc aacataatag gatcttccat

2461 gttggaagca gaacaaaagt tagagtacat tctagaagaa tcaacccact taccatccag

2521 tactgtatca gctgacacta aaccgttggt tggagttatc atgggttcag actctgatct

2581 acctgtgatt tcgaaaggtt gcgatatttt aaaacagttt ggtgttccat tcgaagttac

2641 tattgtctct gctcatagaa caccacagag aatgaccaga tatgcctttg aagccgctag

2701 tagaggtatc aaggctatca ttgcaggtgc tggtggtgct gctcatcttc caggaatggt

2761 tgctgccatg actccgttgc cagtcattgg tgttcctgtc aagggctcta cgttggatgg

2821 tgtagactcg ctacactcga ttgtccaaat gcctagaggt gttcctgtgg ctacggttgc

2881 tatcaacaac gccaccaatg ccgctctgtt ggccatcagg attttaggta caattgacca

2941 caaatggcaa aaggaaatgt ccaagtatat gaatgcaatg gagaccgaag tgttggggaa

3001 ggcatccaac ttggaatctg aagggtatga atcctatttg aagaatcgtc tttgaattta

3061 gtattgtttt ttaatagatg tatatataat agtacacgta acttatctat tccattcata

3121 attttatttt aaaggttcgg tagaaatttg tcctccaaaa agttggttag agcctggcag

3181 ttttgatagg cattattata gattgggtaa tatttaccct gcacctggag gaactttgca

3241 aagagcctca tgtgcggcgc gccaggccat aatggccaaa cggtttctca attactatat

3301 actactaacc atttacctgt agcgtatttc ttttccctct tcgcgaaagc tcaagggcat

3361 cttcttgact catgaaaaat atctggattt cttctgacag atcatcaccc ttgagcccaa

3421 ctctctagcc tatgagtgta agtgatagtc atcttgcaac agattatttt ggaacgcaac

3481 taacaaagca gatacaccct tcagcagaat cctttctgga tattgtgaag aatgatcgcc

3541 aaagtcacag tcctgagaca gttcctaatc tttaccccat ttacaagttc atccaatcag

3601 acttcttaac gcctcatctg gcttatatca agcttaccaa cagttcagaa actcccagtc

3661 caagtttctt gcttgaaagt gcgaagaatg gtgacaccgt tgacaggtac acctttatgg

3721 gacattcccc cagaaaaata atcaagactg ggcctttaga gggtgctgaa gttgacccct

3781 tggtgcttct ggaaaaagaa ctgaagggca ccagacaagc gcaacttcct ggtattcctc

3841 gtctaagtgg tggtgccata ggatacatct cgtacgattg tattaagtac tttgaaccaa

3901 aaactgaaag aaaactgaaa gatgttttgc aacttccgga agcagctttg atgttgttcg

3961 acacgatcgt ggcttttgac aatgtttatc aaagattcca ggtaattgga aacgtttctc

4021 tatccgttga tgactcggac gaagctattc ttgagaaata ttataagaca agagaagaag

4081 tggaaaagat cagtaaagtg gtatttgaca ataaaactgt tccctactat gaacagaaag

4141 atattattca aggccaaacg ttcacctcta atattggtca ggaagggtat gaaaaccatg

4201 ttcgcaagct gaaagaacat attctgaaag gagacatctt ccaagctgtt ccctctcaaa

4261 gggtagccag gccgacctca ttgcaccctt tcaacatcta tcgtcatttg agaactgtca

4321 atccttctcc atacatgttc tatattgact atctagactt ccaagttgtt ggtgcttcac

4381 ctgaattact agttaaatcc gacaacaaca acaaaatcat cacacatcct attgctggaa

4441 ctcttcccag aggtaaaact atcgaagagg acgacaatta tgctaagcaa ttgaagtcgt

4501 ctttgaaaga cagggccgag cacgtcatgc tggtagattt ggccagaaat gatattaacc

4561 gtgtgtgtga gcccaccagt accacggttg atcgtttatt gactgtggag agattttctc

4621 atgtgatgca tcttgtgtca gaagtcagtg gaacattgag accaaacaag actcgcttcg

4681 atgctttcag atccattttc ccagcaggaa ccgtctccgg tgctccgaag gtaagagcaa

4741 tgcaactcat aggagaattg gaaggagaaa agagaggtgt ttatgcgggg gccgtaggac

4801 actggtcgta cgatggaaaa tcgatggaca catgtattgc cttaagaaca atggtcgtca

4861 aggacggtgt cgcttacctt caagccggag gtggaattgt ctacgattct gacccctatg

4921 acgagtacat cgaaaccatg aacaaaatga gatccaacaa taacaccatc ttggaggctg

4981 agaaaatctg gaccgatagg ttggccagag acgagaatca aagtgaatcc gaagaaaacg

5041 atcaatgaac ggaggacgta agtaggaatt tatggtttgg ccataatggc ctagcttggc

5101 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa

5161 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac

5221 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca

5281 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc

5341 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc

5401 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc

5461 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag

5521 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc

5581 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt

5641 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct

5701 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg

5761 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct

5821 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat

5881 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg

5941 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa

6001 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt

6061 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc

6121 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt

6181 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta

6241 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat

6301 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac

6361 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg

6421 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag

6481 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt

6541 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt

6601 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt

6661 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt

6721 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct

6781 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt

6841 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac

6901 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa

6961 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa

7021 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca

7081 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct

7141 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga

7201 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc

7261 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag

7321 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc

7381 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc

7441 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt

7501 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg

7561 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc

7621 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt

7681 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tg

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