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pLysS质粒

货号：kl-zl-0888-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0888-01	pLysS质粒	NA		立即咨询	+ -

产品介绍
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pLysS质粒货号：kl-zl-0888 规格：20ul

启动子：	T7 Phi-3.8
复制子：	p15 ori
质粒分类：	有毒蛋白表达配套质粒
质粒大小：	4886bp
质粒标签：	T7 lysozyme
原核抗性：	氯霉素Chloramphenicol
克隆菌株：	DH5α
培养条件：	37℃，有氧，LB
表达宿主：	大肠杆菌
5'测序引物：	根据序列设计
3'测序引物：	根据序列设计
备注：	低拷贝

质粒简介：
pLysS and pLysE are 4886bp plasmids constructed by insertion of the T7 lysozyme gene into the BamH I site of pACYC184 (1, 2). These plasmids are not cloning vectors; they are used in λDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase. The two plasmids differ only by the orientation of the T7 lysozyme gene. In pLysS the T7 lysozyme coding sequence is in the antisense orientation relative to the tet promoter, so only a small amount of T7 lysozyme is produced. In pLysE large amounts of T7 lysozyme are produced from the tet promoter. The construct also contains the weak T7 φ3.8 promoter immediately following the lysozyme gene. The p15A origin of replication is compatible with those found in pBR322- and pUC-derived plasmids.Unique sites are shown on the circle map.
1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.
2. Chang, A.C.Y. and Cohen, S.N. (1978) J. Bacteriol. 134, 1141.
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