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YEP352质粒

  • 货号:kl-zl-0860-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0860-01 YEP352质粒 NA

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YEP352质粒 货号:kl-zl-0860 规格:20ul

启动子: URA3
复制子: 2μ ori,ori
质粒分类: 酵母系列,酿酒酵母表达载体
质粒大小: 5881bp
原核抗性: Amp
筛选标记: URA3
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 酵母细胞
5'测序引物: M13F:TGTAAAACGACGGCCAGT
3'测序引物: 根据序列设计引物
质粒简介:
Restriction digests of the clone give the following sizes (kb): BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3.This is a Escherichia coli/yeast episomal shuttle vector with multiple restriction sites for cloning.Constructed by filling in and blunt-end ligating pUC18 linearized at NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI site in URA3 were removed.The yeast genomicsequence is in nt 286-1383 (where the A of the ATG of lacZ' is number 1), the 2 micron sequence goes from nt 1384-2768, and pUC18 includes nt 2769-285.
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