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pRS425质粒

  • 货号:kl-zl-0840-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0840-01 pRS425质粒 NA

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pRS425质粒 货号:kl-zl-0840 规格:20ul

启动子: LEU2,T7,T3
复制子: 2μ ori,ori,FI ori
质粒分类: 酵母系列,酿酒酵母表达载体
质粒大小: 6849bp
原核抗性: Amp
筛选标记: LEU2
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 酵母细胞
诱导方式: 半乳糖诱导
5'测序引物: M13R:CAGGAAACAGCTATGACC
3'测序引物: 根据序列设计引物
质粒简介:
This is a YEtype(episomal) yeast shuttle vector. It encodes the beta galactosidase alpha peptide for bluewhite color detection of inserts and also has the T7 and T3 promoters for in vitro RNA synthesis and priming sites for sequencing. It also contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the copy number is about 20 per haploid cell. In nonselective growth, plasmids are lost through mitotic segregation at rates in the range of 4.6 +/ 0.6% of progeny per doubling. The order of the major features in this plasmid is: ori(f1) lacZ T7 promoter MCS (KpnISacI) T3 promoter lacI ori(pMB1) ampR ori(2 micron) LEU2
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