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pROKII质粒

  • 货号:kl-zl-0837-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0837-01 pROKII质粒 NA

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pROKII质粒 货号:kl-zl-0837 规格:20ul

启动子: CaMV 35S
复制子: ori
终止子: NOS
质粒分类: 植物系列,蛋白过表达载体
质粒大小: 12880bp
原核抗性: Kan
筛选标记: Neo
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
5'测序引物: 35S:GACGCACAATCCCACTATCC
3'测序引物: 根据序列设计引物
质粒简介:
An intron sequence of Poptr;CYCD1;1 from Populus trichocarpa was ligated into binary vector,pROKⅡ, to obtain the construct for gene silencing in plants. The resulting pRNAi was used to silence the exogenous fusion gene, GUS:GFP, in transgenic tobacco transformed with pCAMBIA-1303 vector. The specific sequence from GUS and GFP genes was cloned into pRNAi, respectively, and both of them reduced the expression of GUS:GFP gene. The results implied that the present pRNAi could be used to silence some gene of interest.
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