pTRE2pur质粒 货号：kl-zl-0770 规格：20ul
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启动子： |
Tet-responsive promoter |
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复制子： |
pUC ori |
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终止子： |
SV40 poly(A) signal，beta-globin polyA signal |
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质粒分类： |
哺乳细胞，四环素调控系统载体 |
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质粒大小： |
5108bp |
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原核抗性： |
Amp |
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克隆菌株： |
DH5α |
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培养条件： |
37℃，有氧 LB |
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表达宿主： |
哺乳细胞 |
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诱导方式： |
四环素诱导 |
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5'测序引物： |
Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
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3'测序引物： |
根据序列设计引物 |
质粒简介：
pTRE2pur is a response plasmid that expresses a gene of interest (Gene X) in Clontech's Tet-On® and Tet-Off® Gene Expression Systems and Tet-On and Tet-Off Cell Lines (1). The Tet Expression Systems and Cell Lines give researchers ready access to the tetracycline-regulated expression systems described by Gossen & Bujard (2; Tet-Off) and Gossen et al. (3; Tet-On).pTRE2pur contains an MCS immediately downstream of the Tet-responsive PhCMV*-1 promoter. cDNAs or genes inserted into the MCS will be responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively. PhCMV*-1 contains the Tet response element (TRE), which consists of seven copies of the 19-bp tet operator sequence (tetO). TheTRE element is just upstream of the minimal CMV promoter (Pmin CMV), which lacks the enhancer that is part of the complete CMV promoter. Consequently, PhCMV*-1 is silent in the absence of binding of TetR or rTetR to the tetO sequences. Note that the cloned insert must have an initiation codon. In some cases the addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. The puromycin resistance gene is used to directly select for stable transformants.
质粒图谱：
