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pCas质粒

  • 货号：kl-zl-0329-01
  • 培养条件：37℃，有氧，LB
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0329-01 pCas质粒 NA

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pCas质粒 货号：kl-zl-0329
规格：20ul
质粒描述：

启动子： RNR2 promoter
质粒大小： 8761bp
原核抗性： 卡那霉素Kan
克隆菌株： DH5α等大肠杆菌
培养条件： 37℃，有氧，LB
质粒简介：
Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast.
质粒图谱：

序列：
ORIGIN
1 gtatatgtgt tatgtagtat actctttctt caacaattaa atactctcgg tagccaagtt
61 ggtttaaggc gcaagactgt aatttatcac tacgaaatct tgagatcggg cgttcgactc
121 gcccccggga gagatggccg gcatggtccc agcctcctcg ctggcgccgg ctgggcaaca
181 ccttcgggtg gcgaatggga ctttcataat aacgtccaat aaatgtttta gagctagaaa
241 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
301 tttttttatt ttttgtcact attgttatgt aaaatgccac ctctgacagt atggaacgca
361 aacttctgtc tagtggatat aggtctagag atctgtttag cttgcctcgt ccccgccggg
421 tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag
481 ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc
541 atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct
601 gcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc
661 gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact
721 tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg
781 ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt ccaacatgga tgctgattta
841 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg
901 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
961 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
1021 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggc
1081 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
1141 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
1201 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
1261 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
1321 aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
1381 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
1441 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
1501 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
1561 catttgatgc tcgatgagtt tttctaatca gtactgacaa taaaaagatt cttgttttca
1621 agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta
1681 gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc
1741 agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc
1801 gatactaacg ccgccatcca gtgtcgaaaa cgagctctcg agaaccctta atgtcgacag
1861 tcgaacaaga agcaggcaaa gtttagagca ctgcccctcc gcactcaaaa aagaaaaaac
1921 taggaggaaa ataaaattct caaccacaca aacacataaa cacatacaaa tacaaataca
1981 agcttattta cttgacatcg cgcgatcttc cactattcag cgccgtccgc cctctctcgt
2041 gttttttgtt tacgcgacaa ctatgcgaaa tccggagcaa cgggcaaccg tttggggaaa
2101 gaccacaccc acgcgcgatc gccatggcaa cgaggtcgca cacgccccac acccagacct
2161 ccctgcgagc gggcatgggt acaatgtccc cgttgccaca gacaccactt cgtagcacag
2221 cgcagagcgt agcgtgttgt tgctgctgac aaaagaaaat ttttcttagc aaagcaaagg
2281 aggggaagca cgggcagata gcaccgtacc atacccttgg aaactcgaaa tgaacgaagc
2341 aggaaatgag agaatgagag ttttgtaggt atatatagcg gtagtgtttg cgcgttacca
2401 tcatcttctg gatctatcta ttgttctttt cctcatcact ttcccctttt tcgctcttct
2461 tcttgtcttt tatttctttc ttttttttaa ttgttccctc gattggctat ctaccaaaga
2521 atccaaactt aatacacgta tttatttgtc caattaccat ggataagaaa tactcaatag
2581 gcttagatat cggcacaaat agcgtcggat gggcggtgat cactgatgaa tataaggttc
2641 cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa aaaaatctta
2701 taggggctct tttatttgac agtggagaga cagcggaagc gactcgtctc aaacggacag
2761 ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag attttttcaa
2821 atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct tttttggtgg
2881 aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat gaagttgctt
2941 atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat tctactgata
3001 aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt cgtggtcatt
3061 ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta tttatccagt
3121 tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt ggagtagatg
3181 ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaaaat ctcattgctc
3241 agctccccgg tgagaagaaa aatggcttat ttgggaatct cattgctttg tcattgggtt
3301 tgacccctaa ttttaaatca aattttgatt tggcagaaga tgctaaatta cagctttcaa
3361 aagatactta cgatgatgat ttagataatt tattggcgca aattggagat caatatgctg
3421 atttgttttt ggcagctaag aatttatcag atgctatttt actttcagat atcctaagag
3481 taaatactga aataactaag gctcccctat cagcttcaat gattaaacgc tacgatgaac
3541 atcatcaaga cttgactctt ttaaaagctt tagttcgaca acaacttcca gaaaagtata
3601 aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat gggggagcta
3661 gccaagaaga attttataaa tttatcaaac caattttaga aaaaatggat ggtactgagg
3721 aattattggt gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg
3781 gctctattcc ccatcaaatt cacttgggtg agctgcatgc tattttgaga agacaagaag
3841 acttttatcc atttttaaaa gacaatcgtg agaagattga aaaaatcttg acttttcgaa
3901 ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga
3961 agtctgaaga aacaattacc ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag
4021 ctcaatcatt tattgaacgc atgacaaact ttgataaaaa tcttccaaat gaaaaagtac
4081 taccaaaaca tagtttgctt tatgagtatt ttacggttta taacgaattg acaaaggtca
4141 aatatgttac tgaaggaatg cgaaaaccag catttctttc aggtgaacag aagaaagcca
4201 ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt taagcaatta aaagaagatt
4261 atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc aggagttgaa gatagattta
4321 atgcttcatt aggtacctac catgatttgc taaaaattat taaagataaa gattttttgg
4381 ataatgaaga aaatgaagat atcttagagg atattgtttt aacattgacc ttatttgaag
4441 atagggagat gattgaggaa agacttaaaa catatgctca cctctttgat gataaggtga
4501 tgaaacagct taaacgtcgc cgttatactg gttggggacg tttgtctcga aaattgatta
4561 atggtattag ggataagcaa tctggcaaaa caatattaga ttttttgaaa tcagatggtt
4621 ttgccaatcg caattttatg cagctgatcc atgatgatag tttgacattt aaagaagaca
4681 ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca tgaacatatt gcaaatttag
4741 ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt gatgaattgg
4
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