pTRE2hyg质粒

pTRE2hyg质粒 货号：kl-zl-0223

pTRE2hyg is a response plasmid that expresses a gene of interest (Gene X) in CLONTECH's Tet-On™ and Tet-Off™ Gene Expression Systems and Tet-On and Tet-Off Cell Lines (1). The Tet Expression Systems and Cell Lines give researchers ready access to the tetracycline-regulated expression systems described by Gossen & Bujard (2; Tet-Off) and Gossenet al. (3; Tet-On). pTRE2hyg contains an MCS immediately downstream of the Tet-responsiveP_hCMV*-1promoter. cDNAs or genes inserted into the MCS will be responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively.P_hCMV*-1contains the Tet response element (TRE), which consists of seven copies of the 19-bp tet operator sequence (tetO). The TRE element is just upstream of the minimal CMV promoter (P_{min CMV}), which lacks the enhancer that is part of the complete CMV promoter. Consequently,P_hCMV*-1is silent in the absence of binding of TetR or rTetR to thetetOsequences. Note that the cloned insert must have an initiating ATG codon. In some cases, addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. pTRE2hyg also contains the hygromycin resistance gene for direct selection of stable transformants. The parental vector pTRE2 was originally described as pUHD10-3 in reference 5.

The pTRE2hyg-Luc Control Vector, packaged with the pTRE2hyg Vector, contains an additional 1649 bp encoding firefly luciferase inserted into the MCS. This vector can be used as a reporter of induction efficiency using standard luciferase detection reagents. It is not intended as a cloning vector.
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