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pECMV-3×FLAG-MC2R (NM_000529)人源哺乳表达质粒

货号：KL-Z1988-01
培养条件：37℃ 二,质粒简介

货号	产品名称	规格	库存	价格	数量	购买
KL-Z1988-01	pECMV-3×FLAG-MC2R (NM_000529)人源哺乳表达质粒	NA		立即咨询	+ -

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pECMV-3×FLAG-MC2R (NM_000529)人源哺乳表达质粒货号：KL-Z1988 规格：5ug
一,质粒描述：

原核抗性：氨苄青霉素Amp

筛选标记：遗传霉素G418

克隆菌株：大肠杆菌DH5α

培养条件： 37℃
二,质粒简介：
pECMV-3×FLAG-MC2R (NM_000529)人源基因哺乳表达质粒
三,质粒图谱及序列：

CDS complement(5350..6210)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/note="AmpR"

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(6211..6315)

/gene="bla"

/note="AmpR promoter"

ORIGIN

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc

901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac

961 atcgactaca aggatgacga tgacaagctt ggtaccgagc tcggatccat gaagcacatt

1021 atcaactcgt atgaaaacat caacaacaca gcaagaaata attccgactg tcctcgtgtg

1081 gttttgccgg aggagatatt tttcacaatt tccattgttg gagttttgga gaatctgatc

1141 gtcctgctgg ctgtgttcaa gaataagaat ctccaggcac ccatgtactt tttcatctgt

1201 agcttggcca tatctgatat gctgggcagc ctatataaga tcttggaaaa tatcctgatc

1261 atattgagaa acatgggcta tctcaagcca cgtggcagtt ttgaaaccac agccgatgac

1321 atcatcgact ccctgtttgt cctctccctg cttggctcca tcttcagcct gtctgtgatt

1381 gctgcggacc gctacatcac catcttccac gcactgcggt accacagcat cgtgaccatg

1441 cgccgcactg tggtggtgct tacggtcatc tggacgttct gcacggggac tggcatcacc

1501 atggtgatct tctcccatca tgtgcccaca gtgatcacct tcacgtcgct gttcccgctg

1561 atgctggtct tcatcctgtg cctctatgtg cacatgttcc tgctggctcg atcccacacc

1621 aggaagatct ccaccctccc cagagccaac atgaaagggg ccatcacact gaccatcctg

1681 ctcggggtct tcatcttctg ctgggccccc tttgtgcttc atgtcctctt gatgacattc

1741 tgcccaagta acccctactg cgcctgctac atgtctctct tccaggtgaa cggcatgttg

1801 atcatgtgca atgccgtcat tgaccccttc atatatgcct tccggagccc agagctcagg

1861 gacgcattca aaaagatgat cttctgcagc aggtactggt agctcgagtc tagagggccc

1921 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc

1981 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa

2041 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg

2101 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg

2161 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg

2221 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca

2281 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc

2341 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct

2401 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg

2461 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc

2521 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg

2581 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg

2641 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag

2701 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag

2761 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc

2821 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc

2881 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat

2941 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag

3001 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg

3061 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg

3121 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg

3181 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg

3241 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg

3301 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc

3361 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc

3421 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc

3481 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc

3541 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg

3601 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct

3661 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt

3721 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc

3781 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt

3841 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc

3901 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg

3961 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc

4021 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac

4081 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc

4141 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct

4201 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat

4261 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc

4321 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg

4381 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct

4441 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt

4501 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc

4561 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga

4621 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata

4681 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac

4741 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg

4801 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc

4861 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag

4921 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt

4981 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt

5041 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg

5101 atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg

5161 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg

5221 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta

5281 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg

5341 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg

5401 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc

5461 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc

5521 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc

5581 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag

5641 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat

5701 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg

5761 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt

5821 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag

5881 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg

5941 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt

6001 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct

6061 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac

6121 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat

6181 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat

6241 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca

6301 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
图谱：

原核抗性	氨苄青霉素Amp
筛选标记	遗传霉素G418
克隆菌株	大肠杆菌DH5α
培养条件	37℃ 二,质粒简介

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