HT115(DE3)线虫实验用大肠杆菌

基因型：

E. coli [F-, mcrA, mcrB, IN(rrnD-rrnE)1, rnc14：：Tn10(DE3 lysogen： lavUV5 promoter -T7 polymerase].

基本信息：

抗性：Tet

培养基：LB

菌株类别：大肠杆菌

培养条件：37℃，有氧，LB

质粒转化：42℃热激

保存方式：20%甘油，-20℃

基本应用：用于线虫实验

备注：

菌株简介：

HT115(DE3) 是一个RNAi Feeding 菌株，抗性是四环素Tet，可用37℃，LB，有氧的方式培养，主要用于线虫的干扰实验。

HT115 is an Rnase III-deficient E. coli strain with IPTG-inducible T7 Polymerase activity. To induce dsRNA production from these plasmids, the HT115 bacteria is grown on special RNAi NGM feeding plates that contain IPTG and the ampicillin analog carbenicillin. Carbenicillin is preferred over ampicillin because it tends to be more stable.

Bacterial strain. Genotype： F-, mcrA, mcrB, IN(rrnD-rrnE)1, rnc14：：Tn10(DE3 lysogen： lavUV5 promoter -T7 polymerase) (IPTG-inducible T7 polymerase) (RNAse III minus). This strain grows on LB or 2XYT plates. This strain is tetracycline esistant. Researchers using this strain should test for expression by transforming in one of the plasmids from the Fire Vector Kit (1999) (pLT76, e.g.) using standard CaCl2 transformation techniques. Biosafety Level： BSL-1.

Bacteria-mediated RNAi--General outline：