pAC5.1b-EGFP-2昆虫系列质粒

pAC5.1b-EGFP② is a 6kb expression vector designed for use with the Drosophila Constitutive Expression System available from Invitrogen. Upon transfection, the vector allows transient expression of your protein of interest in Drosophila cells. When cotransfected with the selection vector, pCoHygro or pCoBlast, pAC5.1b-EGFP allows selection of stable cell lines exhibiting constitutive expression of the protein of interest. • The Drosophila actin 5C (Ac5) promoter for high-level, constitutive expression of the gene of interest in S2 cells (Chung and Keller, 1990). • Multiple cloning site to facilitate cloning the gene of interest. • C-terminal peptide containing the V5 epitope and polyhistidine (6xHis) tag for detection and purification of your protein of interest (if desired). • Three reading frames to facilitate in-frame cloning with the C-terminal peptide. • N-terminal EGFP tag for better detection. • Ampicillin resistance gene for selection of transformants in E. coli.

pAC5.1b-EGFP-2质粒是一个昆虫细胞组成型表达载体，Ac5启动子驱动EGFP标签、目的基因、Myc标签和6×HIS标签一起融合表达，可以与筛选载体pCoHygro或者pCoBlast共转染，建立稳表达细胞系。