pESC-HIS酵母系列载体

  • 货号:KL-ZL3179-3ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL3179-3ug pESC-HIS酵母系列载体 3ug

立即咨询

+ -
货号 名称 规格
KL-ZL3179 pESC-HIS酵母系列载体

3ug

pESC-HIS酵母系列载体
The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae. Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter. These vectors also feature an extensive polylinker sequence and the ability to generate end-specific RNA transcripts from T3 and T7 promoters. Each of the pESC vectors contains one of four different yeast-selectable markers (HIS3, TRP1, LEU2, or URA3) in the same vector backbone. The pESC vectors contain DNA sequences coding for epitope peptides that can be specifically recognized by monoclonal antibodies. A sequence for the FLAG® epitope (DYKDDDDK) is located in the multiple cloning site (MCS) downstream of the GAL10 promoter; a sequence for the c-Myc epitope (EQKLISEEDL) is located in the MCS downstream of the GAL1 promoter. You can insert your gene of interest in front of the epitope sequence to generate C-terminal tagging or after the epitope sequence for N-terminal tagging. These tags allow the protein of interest to be studied without generating a specific antibody to that protein. The epitope-tagged fusion proteins can be studied in transformed cells using well-characterized antibodies. • Express two different genes simultaneously in S. cerevisiae • Proteins tagged with unique epitope tags • Fast and easy immunoprecipitations
启动子: GAL1,GAL10 promoter
质粒大小: 6706bp
筛选标记: DH5α
克隆菌株: 37℃,有氧,LB

序列:
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
1921 gatcggtgcg ggcctcttcg ctattacgcc agctgaattg gagcgacctc atgctatacc
1981 tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa
2041 acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta tttaataata
2101 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta tctaccaacg
2161 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg
2221 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctca gatcttatcg
2281 tcgtcatcct tgtaatccat cgatactagt gcggccgccc tttagtgagg gttgaattcg
2341 aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa taatcatatt
2401 acatggcatt accaccatat acatatccat atacatatcc atatctaatc ttacttatat
2461 gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc tttggaactt
2521 tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag ccgccgagcg
2581 ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt
2641 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact
2701 agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat
2761 gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag ccttatttct
2821 ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat aaatgcaaaa
2881 actgcataac cactttaact aatactttca acattttcgg tttgtattac ttcttattca
2941 aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga
3001 gaaaaaaccc cggatccgta atacgactca ctatagggcc cgggcgtcga catggaacag
3061 aagttgattt ccgaagaaga cctcgagtaa gcttggtacc gcggctagct aagatccgct
3121 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata
3181 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga
3241 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg
3301 aagatccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
3361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
3421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
3481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
3541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
3601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
3661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
3721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
3781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
3841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
3901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
3961 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
4021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
4081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
4141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
4201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
4261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
4321 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
4381 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
4441 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
4501 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
4561 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
4621 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
4681 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
4741 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
4801 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
4861 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
4921 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
4981 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
5041 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
5101 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
5161 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
5221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
5281 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
5341 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
5401 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
5461 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
5521 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
5581 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
5641 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
5701 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
5761 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
5821 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
5881 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
5941 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
6001 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
6061 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
6121 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
6181 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
6241 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
6301 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
6361 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
6421 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
6481 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
6541 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
6601 ctcatccttc aatgctatca tttcctttga tattggatca tctaagaaac cattattatc
6661 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc


货号 产品名称 规格 库存 价格 数量 购买
KL-ZL1661-2ug pML107酵母编辑质粒 2ug

立即咨询

+ -
KL-ZL3433-3ug p415-GalL-Cas9-CYC1t酵母编辑质粒 3ug

立即咨询

+ -
KL-ZL1677-2ug pPIC9K-His毕赤酵母表达质粒 2ug

立即咨询

+ -
KL-ZL3328-3ug YCPlac33酿酒酵母质粒 3ug

立即咨询

+ -
KL-pPR3-C-2ug pPR3-C酵母杂交质粒 2ug

立即咨询

+ -
KL-ZL1619-2ug pY26TEF-GPD酵母双框质粒 2ug

立即咨询

+ -
KL-ZL3369-3ug pDUAL-HFG1酿酒酵母质粒 3ug

立即咨询

+ -
KL-ZL1660-2ug pML104酵母编辑质粒 2ug

立即咨询

+ -
KL-ZL2282-2ug PPICZA-EGFP毕赤酵母质粒 2ug

立即咨询

+ -
KL-ZL1810-5ug p414-TEF1p-Cas9-CYC1t酵母编辑Cas9表达质粒 5ug

立即咨询

+ -
KL-ZL1808-5ug pLacZi酿酒酵母单杂交质粒 5ug

立即咨询

+ -
KL-ZL1662-2ug pJH001酵母编辑质粒 2ug

立即咨询

+ -