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pRS315-TEF-CYC1酿酒酵母质粒

货号：KL-ZL3334-3ug

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL3334-3ug	pRS315-TEF-CYC1酿酒酵母质粒	3ug		立即咨询	+ -

产品介绍
相关产品

》产品信息

货号	名称	规格
KL-ZL3334	pRS315-TEF-CYC1酿酒酵母质粒	3ug

》产品描述

原核抗性： Amp 筛选标记： LEU2 克隆菌株： DH5α 培养条件： LB/37℃

》产品简介

pRS315-TEF-CYC1酿酒酵母质粒

》产品图谱及序列

序列：

promoter complement(5948..6052)

/gene="bla"

/note="AmpR promoter"

misc_feature 6089..6592

/note="CEN/ARS"

/note="S. cerevisiae CEN6 centromere fused to an

autonomously replicating sequence"

ORIGIN

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc

241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca

301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat

361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc

421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc

481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt

541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg

601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct

661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg

721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct

781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac

841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat

901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc

961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg

1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca

1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc

1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata

1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact

1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc

1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca

1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt

1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca

1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg

1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca

1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga

1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc

1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata

1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat

1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat

1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct

1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca

2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat

2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga

2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg

2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt

2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg

2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta

2401 gaagttctcc tcgagggtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa

2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt

2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa

2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag

2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt

2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac

2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag

2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg

2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg

2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc

3001 cagctggcga aggggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc

3061 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa

3121 ttggagctcc atagcttcaa aatgtttcta ctcctttttt actcttccag attttctcgg

3181 actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa atttcccctc

3241 tttcttcctc tagggtgtcg ttaattaccc gtactaaagg tttggaaaag aaaaaagaga

3301 ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt

3361 tcttgaaaat tttttttttg atttttttct ctttcgatga cctcccattg atatttaagt

3421 taataaacgg tcttcaattt ctcaagtttc agtttcattt ttcttgttct attacaactt

3481 tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt ttctagaact

3541 agcgcggccg ctctagaact agtggatccc ccgggctgca ggaattcgat atcaagctta

3601 tcgataccgt cgactcatgt aattagttat gtcacgctta cattcacgcc ctccccccac

3661 atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt

3721 ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct

3781 gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg

3841 acgctcgaag gctttaattt gcgggcccgg tacccagctt ttgttccctt tagtgagggt

3901 taattccgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc

3961 tcacaattcc acacaacata ggagccggaa gcataaagtg taaagcctgg ggtgcctaat

4021 gagtgaggta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc

4081 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg

4141 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag

4201 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag

4261 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc

4321 tggcgttttt ccataggctc ggcccccctg acgagcatca caaaaatcga cgctcaagtc

4381 agaggtggcg aaacccgaca ggactataaa gataccaggc gttcccccct ggaagctccc

4441 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt

4501 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg

4561 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat

4621 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag

4681 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt

4741 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc

4801 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta

4861 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag

4921 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga

4981 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa

5041 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa

5101 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactgc

5161 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga

5221 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa

5281 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt

5341 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg

5401 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc

5461 aacgatcaag gcgagttaca tgatccccca tgttgtgaaa aaaagcggtt agctccttcg

5521 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag

5581 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt

5641 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt

5701 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac

5761 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac

5821 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag

5881 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa

5941 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga

6001 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc

6061 cccgaaaagt gccacctggg tccttttcat cacgtgctat aaaaataatt ataatttaaa

6121 ttttttaata taaatatata aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa

6181 tagtttttgt tttccgaaga tgtaaaagac tctaggggga tcgccaacaa atactacctt

6241 ttatcttgct cttcctgctc tcaggtatta atgccgaatt gtttcatctt gtctgtgtag

6301 aagaccacac acgaaaatcc tgtgatttta cattttactt atcgttaatc gaatgtatat

6361 ctatttaatc tgcttttctt gtctaataaa tatatatgta aagtacgctt tttgttgaaa

6421 ttttttaaac ctttgtttat ttttttttct tcattccgta actcttctac cttctttatt

6481 tactttctaa aatccaaata caaaacataa aaataaataa acacagagta aattcccaaa

6541 ttattccatc attaaaagat acgaggcgcg tgtaagttac aggcaagcga tccgtcctaa

6601 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt

6661 c

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