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pY26TEF-GPD酵母双框质粒

货号：KL-ZL1619-2ug

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL1619-2ug	pY26TEF-GPD酵母双框质粒	2ug		立即咨询	+ -

产品介绍
相关产品

》产品信息

货号	名称	规格
KL-ZL1619	pY26TEF-GPD酵母双框质粒	2ug

》产品描述

启动子： TEF1 promoter，GAP promoter 复制子： 2μ ori，pBR322 ori，F1 ori 终止子： ADH1 terminator，CYC1 terminator 质粒大小： 7430bp 原核抗性：氨苄青霉素Amp 筛选标记： URA3 克隆菌株：大肠杆菌DH5α 培养条件： 37℃，有氧，LB 表达宿主：酿酒酵母

》产品简介

pY26TEF-GPD是一个酿酒酵母高拷贝双向表达质粒，TEF1 promoter，GAP promoter分别驱动两个基因的表达。

》产品图谱及序列

序列：

ORIGIN

1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt

61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg

121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg

181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac

241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata

301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc

361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt

421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa

481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga

541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat

601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct

661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta

721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag

781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc

841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct

901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt

961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg

1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa

1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa

1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta

1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt

1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt

1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt

1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc

1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa

1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc

1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa

1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa

1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg

1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa

1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc

1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc

1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta

1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag

2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca

2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata

2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc

2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca

2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca

2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg

2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa

2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt

2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat

2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg

2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga

2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac

2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt

2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag

2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc

2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag

3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca

3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt

3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc

3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc

3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc

3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa

3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac

3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc

3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa

3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa

3601 agggaacaaa agctggagct cgctattacg ccagctgaat tggagcgacc tcatgctata

3661 ctgagaaagc aacctgacct acaggaaaga gttactcaag aataagaatt ttcgttttaa

3721 aacctaagag tcactttaaa atttgtatac acttattttt tttataactt atttaataat

3781 aaaaatcata aatcataaga aattcgctta tttagaagtg tcaacaacgt atctaccaac

3841 gatttgaccc ttttccatct tttcgtaaat ttctggcaag gtagacaagc cgacaacctt

3901 gattggagac ttgaccaaac ctctggcgaa gaattgttaa ttaaagatct ccgcggtccg

3961 gagttaactg atcagcggcc gcgctagttc tagaaaactt agattagatt gctatgcttt

4021 ctttctaatg agcaagaagt aaaaaaagtt gtaatagaac aagaaaaatg aaactgaaac

4081 ttgagaaatt gaagaccgtt tattaactta aatatcaatg ggaggtcatc gaaagagaaa

4141 aaaatcaaaa aaaaaatttt caagaaaaag aaacgtgata aaaattttta ttgccttttt

4201 cgacgaagaa aaagaaacga ggcggtctct tttttctttt ccaaaccttt agtacgggta

4261 attaacgaca ccctagagga agaaagaggg gaaatttagt atgctgtgct tgggtgtttt

4321 gaagtggtac ggcgatgcgc ggagtccgag aaaatctgga agagtaaaaa aggagtagaa

4381 acattttgaa gctatgagct ccagcttttg ttccctttag tgagggttaa ttgcgcgccg

4441 agctcagttt atcattatca atactcgcca tttcaaagaa tacgtaaata attaatagta

4501 gtgattttcc taactttatt tagtcaaaaa attagccttt taattctgct gtaacccgta

4561 catgcccaaa atagggggcg ggttacacag aatatataac atcgtaggtg tctgggtgaa

4621 cagtttattc ctggcatcca ctaaatataa tggagcccgc tttttaagct ggcatccaga

4681 aaaaaaaaga atcccagcac caaaatattg ttttcttcac caaccatcag ttcataggtc

4741 cattctctta gcgcaactac agagaacagg ggcacaaaca ggcaaaaaac gggcacaacc

4801 tcaatggagt gatgcaacct gcctggagta aatgatgaca caaggcaatt gacccacgca

4861 tgtatctatc tcattttctt acaccttcta ttaccttctg ctctctctga tttggaaaaa

4921 gctgaaaaaa aaggttgaaa ccagttccct gaaattattc ccctacttga ctaataagta

4981 tataaagacg gtaggtattg attgtaattc tgtaaatcta tttcttaaac ttcttaaatt

5041 ctacttttat agttagtctt ttttttagtt ttaaaacacc agaacttagt ttcgacggat

5101 tctagaacta gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc

5161 gacctcgagt catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg

5221 ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta

5281 tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca

5341 gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct

5401 cgaaggcttt aatttgcggc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg

5461 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa

5521 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga

5581 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg

5641 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc

5701 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc

5761 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct

5821 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac

5881 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac

5941 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat

6001 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa

6061 aatattaacg tttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt

6121 tcacaccgca tagggtaata actgatataa ttaaattgaa gctctaattt gtgagtttag

6181 tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc ttctcaaata

6241 tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc ttccctttgc

6301 aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat catccacggt

6361 tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg gtgtcataat

6421 caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa agccgataac

6481 aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc cagtagatag

6541 ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct ttgttacttc

6601 ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt gtgcattcgt

6661 aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt tgactgtatt

6721 accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc

6781 ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca catgtgtttt

6841 tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct tggtggtacg

6901 aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa atagcttggc

6961 agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg acatgattta

7021 tcttcgtttc ctgcaggttt ttgttctgtg cagttgggtt aagaatactg ggcaatttca

7081 tgtttcttca acactacata tgcgtatata taccaatcta agtctgtgct ccttccttcg

7141 ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaagaaacc gaaatcaaaa

7201 aaaagaataa aaaaaaaatg atgaattgaa ttgaaaagct gtggtatggt gcactctcag

7261 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga

7321 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc

7381 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga

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