YCPlac33酿酒酵母质粒

  • 货号:KL-ZL3328-3ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL3328-3ug YCPlac33酿酒酵母质粒 3ug

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货号 名称 规格
KL-ZL3328 YCPlac33酿酒酵母质粒

3ug

原核抗性: Amp 筛选标记: URA3 克隆菌株: DH5α 培养条件: LB/37℃
YCPlac33酿酒酵母质粒

序列:
CDS 3800..4660
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4831..5419
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata atcgctgggc cattctcatg aagaatatct tgaatttatt
541 gtcatattac tagttggtgt ggaagtccta atatcggtga tcaatatagt ggttgacatg
601 ctggctagtc aacattgagc cttttgatca tgcaaatata ttacggtatt ttacaatcaa
661 atatcaaact taactattga ctttataact tatttaggtg gtaacattct tataaaaaag
721 aaaaaaatta ctgcaaaaca gtactagctt ttaacttgta tcctaggtta tctatgctgt
781 ctcaccatag agaatattac ctatttcaga atgtatgtcc atgattcgcc gggtaaatac
841 atataataca caaatctggc ttaataaagt ctataatata tctcataaag aagtgctaaa
901 ttggctagtg ctatatattt ttaagaaaat ttcttttgac taagtccata tcgactttgt
961 aaaagttcac attagcatac atatattaca cgagccagaa atagtaactt ttgcctaaat
1021 cacaaattgc aaaatttaat tgcttgcaaa aggtcacatg cttataatca acttttttaa
1081 aaatttaaaa tactttttta ttttttattt ttaaacataa atgaaataat ttatttattg
1141 tttatgatta ccgaaacata aaacctgctc aagaaaaaga aactgttttg tccttggaaa
1201 aaaagcacta cctaggagcg gccaaaatgc cgaggctttc atagcttaaa ctctttacag
1261 aaaataggca ttatagatca gttcgagttt tcttattctt ccttccggtt ttatcgtcac
1321 agttttacag taaataagta tcacctctta gagttcgatg ataagctgtc aaacatgaga
1381 attaattcca catgttaaaa tagtgaagga gcatgttcgg cacacagtgg accgaacgtg
1441 gggtaagtgc actagggtcc ggttaaacgg atctcgcatt gatgaggcaa cgctaattat
1501 caacatatag attgttatct atctgcatga acacgaaatc tttacttgac gacttgaggc
1561 tgatggtgtt tatgcaaaga aaccactgtg tttaatatgt gtcactgttt gatattactg
1621 tcagcgtaga agataatagt aaaagcggtt aataagtgta tttgagataa gtgtgataaa
1681 gtttttacag cgaaaagacg ataaatacaa gaaaatgatt acgaggatac ggagagaggt
1741 atgtacatgt gtatttatat actaagctgc cggcggttgt ttgcaagacc gagaaaaggc
1801 tagcaagaat cgggtcattg tagcgtatgc gcctgtgaac attctcttca acaagtttga
1861 ttccattgcg gtgaaatggt aaaagtcaac cccctgcgat gtatattttc ctgtacaatc
1921 aatcaaaaag ccaaatgatt tagcattatc tttacatctt gttattttac agattttatg
1981 tttagatctt ttatgcttgc ttttcaaaag gcttgcaggc aagtgcacaa acaatactta
2041 aataaatact actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg
2101 ttagagtctt ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca
2161 tttaatctaa gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt
2221 aagctctcgg ggctctcttg ccttccaacc cagtcagaaa tcgagttcca atccaaaagt
2281 tcacctgtcc cacctgcttc tgaatcaaac aagggaataa acgaatgagg tttctgtgaa
2341 gctgcactga gtagtatgtt gcagtctttt ggaaatacga gtcttttaat aactggcaaa
2401 ccgaggaact cttggtattc ttgccacgac tcatctccat gcagttggac gatcgatgat
2461 aagctgtcaa acatgagaat tgggtaataa ctgatataat taaattgaag ctctaatttg
2521 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
2581 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
2641 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
2701 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
2761 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
2821 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
2881 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
2941 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
3001 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
3061 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
3121 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
3181 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
3241 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
3301 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
3361 catgatttat cttcgtttcc tgcatgtttt tgttctgtgc agttgggtta agaatactgg
3421 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
3481 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaagaaaccg
3541 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat tgaaaagcta attcttgaag
3601 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
3661 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
3721 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
3781 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
3841 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
3901 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
3961 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
4021 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
4081 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
4141 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
4201 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
4261 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
4321 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
4381 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
4441 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
4501 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
4561 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
4621 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
4681 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
4741 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
4801 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
4861 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
4921 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
4981 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
5041 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
5101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
5161 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
5221 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
5281 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
5341 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
5401 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
5461 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
5521 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
5581 agtgagcgag gaagcggaag a

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