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p414-TEF1p-Cas9-CYC1t酵母编辑Cas9表达质粒

货号：KL-ZL1810-5ug

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL1810-5ug	p414-TEF1p-Cas9-CYC1t酵母编辑Cas9表达质粒	5ug		立即咨询	+ -

产品介绍
相关产品

》产品信息

货号	名称	规格
KL-ZL1810	p414-TEF1p-Cas9-CYC1t酵母编辑Cas9表达质粒	5ug

》产品描述

启动子： RPR1 promoter 原核抗性：氨苄青霉素Amp 筛选标记： LEU2 克隆菌株：大肠杆菌DH5α 培养条件： 37℃

》产品简介

pRPR1_gRNA_handle_RPR1t是一个酵母细胞基因编辑gRNA表达质粒。

》产品图谱及序列

序列：

CDS 5937..7031

/codon_start=1

/gene="S. cerevisiae LEU2"

/product="3-isopropylmalate dehydrogenase, required for

leucine biosynthesis"

/label=LEU2

/note="yeast auxotrophic marker"

/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL

IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA

NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT

VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ

LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF

GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG

DLGGSNSTTEVGDAVAEEVKKILA"

ORIGIN

1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt

61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg

121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg

181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac

241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata

301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc

361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt

421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa

481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga

541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat

601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct

661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta

721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag

781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc

841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct

901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt

961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg

1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa

1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa

1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta

1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt

1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt

1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt

1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc

1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa

1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc

1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa

1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa

1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg

1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa

1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc

1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc

1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta

1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag

2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca

2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata

2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc

2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca

2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca

2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg

2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa

2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt

2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat

2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg

2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga

2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac

2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt

2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag

2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc

2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag

3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca

3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt

3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc

3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc

3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc

3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa

3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac

3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc

3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa

3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa

3601 agggaacaaa agctggagct cgggggatct gccaattgaa cataacatgg tagttacata

3661 tactagtaat atggttcggc acacattaaa agtataaaaa ctatctgaat tacgaattac

3721 atatattggt cataaaaatc aatcaatcat cgtgtgtttt atatgtctct tatctaagta

3781 taagaatatc catagttaat attcacttac gctacctttt aacctgtaat cattgtcaac

3841 aggatatgtt aacgacccac attgataaac gctagtattt ctttttcctc ttcttattgg

3901 ccggctgtct ctatactccc ctatagtctg tttcttttcg tttcgattgt tttacgtttg

3961 aggcctcgtg gcgcacatgg tacgctgtgg tgctcgcggc tgggaacgaa actctgggag

4021 ctgcgattgg cagaagcttg ttttagagct agaaatagca agttaaaata aggctagtcc

4081 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tctcgagcca tatccaactt

4141 ccaatttaat ctttcttttt taattttcac ttatttgcga tacagaaaga aaaaagcgat

4201 agtaactatt gaattttgtt tggatttggt tagattagat atggtttctc tttatattta

4261 catgctaaaa atgggctaca ccagagatac ataattagat atatatacgc cagtacacct

4321 tatcggccca agccttgtcc caaggcagcg ttttgttctt ggaaacgctg ccctacacgt

4381 tcgctatgct tcaagaactt ttctgagcac ttcatgatgc atgtttgttc cttattggtt

4441 agctttgatg ttgtgaagtc attgacacag tctgtgaaac atctttctac cagattagag

4501 tacaaacgca tgaaatcctt catttgcttt tgttccacta ctttttggaa ctcttgttgt

4561 tctttggtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt

4621 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat

4681 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag

4741 ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg

4801 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg

4861 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg

4921 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt

4981 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt

5041 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta

5101 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa

5161 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt

5221 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatcgacggt

5281 cgaggagaac ttctagtata tccacatacc taatattatt gccttattaa aaatggaatc

5341 ccaacaatta catcaaaatc cacattctct tcaaaatcaa ttgtcctgta cttccttgtt

5401 catgtgtgtt caaaaacgtt atatttatag gataattata ctctatttct caacaagtaa

5461 ttggttgttt ggccgagcgg tctaaggcgc ctgattcaag aaatatcttg accgcagtta

5521 actgtgggaa tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat

5581 ttttttcctc aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg

5641 actggaaatt ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa

5701 aattgggaga aaaaggaaag gtgagaggcc ggaaccggct tttcatatag aatagagaag

5761 cgttcatgac taaatgcttg catcacaata cttgaagttg acaatattat ttaaggacct

5821 attgtttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc ttacctttta

5881 catttcagca atatatatat atatttcaag gatataccat tctaatgtct gcccctatgt

5941 ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa atcacagccg

6001 aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag ttcgatttcg

6061 aaaatcattt aattggtggt gctgctatcg atgctacagg tgtcccactt ccagatgagg

6121 cgctggaagc ctccaagaag gttgatgccg ttttgttagg tgctgtggct ggtcctaaat

6181 ggggtaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa gaacttcaat

6241 tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac ttatctccaa

6301 tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta gtgggaggta

6361 tttactttgg taagagaaag gaagacgatg gtgatggtgt cgcttgggat agtgaacaat

6421 acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc ctacaacatg

6481 agccaccatt gcctatttgg tccttggata aagctaatct tttggcctct tcaagattat

6541 ggagaaaaac tgtggaggaa accatcaaga acgaattccc tacattgaag gttcaacatc

6601 aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta aatggtatta

6661 taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt atcccaggtt

6721 ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac accgcatttg

6781 gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag gttgacccta

6841 tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg cctgaagaag

6901 gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga actggtgatt

6961 taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa

7021 tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa

7081 ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact

7141 atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatagt aaaggaatac

7201 aggtaagcaa attgatacta atggctcaac gtgataagga aaaagaattg cactttaaca

7261 ttaatattga caaggaggag ggcaccacac aaaaagttag gtgtaacaga aaatcatgaa

7321 actacgattc ctaatttgat attggaggat tttctctaaa aaaaaaaaaa tacaacaaat

7381 aaaaaacact caatgacctg accatttgat ggagtttaag tcaatacctt cttgaagcat

7441 ttcccataat ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg ccttacgacg

7501 tagtcgatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc

7561 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct

7621 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca

7681 ccgaaacgcg cga

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