pRS313-TEF-CYC1酿酒酵母质粒

  • 货号:KL-ZL3329-3ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL3329-3ug pRS313-TEF-CYC1酿酒酵母质粒 3ug

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货号 名称 规格
KL-ZL3329 pRS313-TEF-CYC1酿酒酵母质粒

3ug

原核抗性: Amp 筛选标记: HIS3 克隆菌株: DH5α 培养条件: LB/37℃
pRS313-TEF-CYC1酿酒酵母质粒

序列:
CDS complement(4042..4902)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4903..5007)
/gene="bla"
/note="AmpR promoter"
misc_feature 5044..5547
/note="CEN/ARS"
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga
241 acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt
301 actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt
361 ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa
421 tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca
481 ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa
541 tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc
601 gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat
661 taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc
721 cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac
781 tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctactgg cgcgtggagt
841 aaaaaggttt ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga
901 tctttcgaac aggccgtacg cagttgtcga acttggtttg caaagggaga aagtaggaga
961 tctctcttgc gagatgatcc cgcattttct tgaaagcttt gcagaggcta gcagaattac
1021 cctccacgtt gattgtctgc gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa
1081 ggctcttgcg gttgccataa gagaagccac ctcgcccaat ggtaccaacg atgttccctc
1141 caccaaaggt gttcttatgt agtgacaccg attatttaaa gctgcagcat acgatatata
1201 tacatgtgta tatatgtata cctatgaatg tcagtaagta tgtatacgaa cagtatgata
1261 ctgaagatga caaggtaatg catcattcta tacgtgtcat tctgaacgag gcgcgctttc
1321 cttttttctt tttgcttttt cttttttttt ctcttgaact cgacggatca tatgcggtgt
1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa ggggggatgt gctgcaaggc
1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
2041 aattgtaata cgactcacta tagggcgaat tggagctcca tagcttcaaa atgtttctac
2101 tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca
2161 cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt taattacccg
2221 tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc gtcgaaaaag
2281 gcaataaaaa tttttatcac gtttcttttt cttgaaaatt ttttttttga tttttttctc
2341 tttcgatgac ctcccattga tatttaagtt aataaacggt cttcaatttc tcaagtttca
2401 gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag aaagaaagca
2461 tagcaatcta atctaagttt tctagaacta gcgcggccgc tctagaacta gtggatcccc
2521 cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagt catgtaatta
2581 gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga
2641 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga
2701 acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac
2761 attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcggg
2821 cccggtaccc agcttttgtt ccctttagtg agggttaatt ccgagcttgg cgtaatcatg
2881 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acataggagc
2941 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc
3001 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
3061 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
3121 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
3181 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
3241 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctcggccc
3301 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
3361 ataaagatac caggcgttcc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
3421 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
3481 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3541 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
3601 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
3661 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
3721 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
3781 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
3841 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
3901 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
3961 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
4021 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
4081 ctgtctattt cgttcatcca tagttgcctg actgcccgtc gtgtagataa ctacgatacg
4141 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
4201 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
4261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
4321 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
4381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
4441 ccccatgttg tgaaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
4501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
4561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
4621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
4681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
4741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
4801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
4861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
4921 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
4981 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcctt
5041 ttcatcacgt gctataaaaa taattataat ttaaattttt taatataaat atataaatta
5101 aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa
5161 aagactctag ggggatcgcc aacaaatact accttttatc ttgctcttcc tgctctcagg
5221 tattaatgcc gaattgtttc atcttgtctg tgtagaagac cacacacgaa aatcctgtga
5281 ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt ttcttgtcta
5341 ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg tttatttttt
5401 tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc aaatacaaaa
5461 cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa aagatacgag
5521 gcgcgtgtaa gttacaggca agcgatccgt cctaagaaac cattattatc atgacattaa
5581 cctataaaaa taggcgtatc acgaggccct ttcgtc

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