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pGL3-Basic质粒

货号：kl-zl-1032-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1032-01	pGL3-Basic质粒	NA		立即咨询	+ -

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pGL3-Basic质粒货号：kl-zl-1032 规格：20ul

复制子：	pUC ori,f1 ori
终止子：	SV40 poly(A) signal
质粒分类：	哺乳细胞，信号通路报告载体
质粒大小：	4818bp
原核抗性：	Amp
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	RVP3：CTAGCAAAATAGGCTGTCCC
3'测序引物：	根据序列设计引物

质粒简介：
The pGL3 Luciferase Reporter Vectors(a–c) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.
质粒图谱：

序列：

misc_feature 4720..4811

/note="pause site"

/note="RNA polymerase II transcriptional pause signal from

the human?alpha-2 globin gene"

ORIGIN

1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg

61 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg

121 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag

181 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc

241 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg

301 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg

361 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa

421 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag

481 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc

541 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat

601 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca

661 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct

721 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc

781 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact

841 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag

901 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta

961 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa

1021 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc

1081 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt

1141 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg

1201 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt

1261 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg

1321 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac

1381 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc

1441 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt

1501 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag

1561 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag

1621 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac

1681 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa

1741 ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt

1801 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct

1861 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt

1921 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc

1981 tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag ccttcaaccc

2041 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt

2101 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg

2161 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa

2221 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc

2281 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc

2341 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat

2401 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc

2461 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct

2521 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg

2581 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc

2641 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga

2701 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa

2761 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta

2821 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc

2881 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg

2941 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga

3001 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg

3061 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct

3121 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg

3181 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc

3241 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa

3301 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc

3361 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt

3421 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc

3481 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt

3541 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc

3601 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt

3661 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata

3721 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga

3781 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag

3841 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa

3901 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt

3961 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga

4021 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct

4081 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg

4141 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg

4201 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac

4261 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct

4321 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt

4381 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt

4441 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt

4501 ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc aactgttggg

4561 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa

4621 gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt tattttcatt

4681 acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa

4741 caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag

4801 aacatttctc tatcgata

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