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pIRES2-EGFP质粒

货号：kl-zl-1052-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1052-01	pIRES2-EGFP质粒	NA		立即咨询	+ -

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pIRES2-EGFP质粒货号：kl-zl-1052 规格：20ul

启动子：	CMV
复制子：	pUC ori,f1 ori
终止子：	SV40 poly(A) signal
质粒分类：	哺乳细胞，荧光蛋白报告载体
质粒大小：	5308bp
原核抗性：	Kan
筛选标记：	Neo
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物：	pEGFP-N-3：CGTCGCCGTCCAGCTCGACCAG

质粒简介：
pIRES2-EGFP contains the internal ribosome entry site (IRES; 1, 2) of the encephalomyocarditis virus (ECMV) between the MCS and the enhanced green fluorescent protein (EGFP) coding region.This permits both the gene of interest (cloned into the MCS) and the EGFP gene to be translated from a single bicistronic mRNA. pIRES2-EGFP is designed for the efficient selection (by flow cytometry or other methods) of transiently transfected mammalian cells expressing EGFP and the protein of interest. This vector can also be used to express EGFP alone or to obtain stably transfected cell lines without time-consuming drug and clonal selection.
质粒图谱：

序列：

polyA_signal 4230..4277

/note="HSV TK poly(A) signal"

/note="HSV TK poly(A) signal;herpesvirus thymidine kinase

polyadenylation signal"

rep_origin 4606..5194

/direction=RIGHT

/note="ori"

/note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

ORIGIN

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg

661 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg

721 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag

781 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc

841 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg

901 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag

961 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca

1021 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt

1081 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc

1141 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa

1201 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga

1261 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg

1321 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc

1381 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga

1441 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg

1501 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg

1561 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc

1621 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg

1681 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca

1741 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact

1801 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga

1861 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg

1921 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc

1981 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa

2041 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt

2101 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa

2161 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg

2221 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct

2281 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg

2341 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact

2401 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac

2461 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga

2521 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga

2581 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca

2641 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa

2701 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca

2761 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg

2821 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc

2881 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc

2941 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt

3001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc

3061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct

3121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag

3181 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg

3241 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg

3301 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc

3361 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga

3421 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc

3481 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag

3541 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat

3601 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg

3661 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca

3721 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct

3781 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg

3841 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg

3901 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc

3961 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat

4021 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta

4081 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg

4141 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga

4201 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg

4261 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac

4321 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc

4381 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat

4441 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag

4501 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc

4561 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt

4621 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt

4681 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat

4741 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc

4801 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa

4861 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg

4921 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag

4981 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag

5041 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa

5101 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt

5161 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg

5221 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc

5281 tgtggataac cgtattaccg ccatgcat

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