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pCDNA3.1-His B质粒

货号：kl-zl-0190-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0190-01	pCDNA3.1-His B质粒	NA		立即咨询	+ -

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pCDNA3.1-His B质粒货号：kl-zl-0190

质粒类型：	哺乳细胞表达载体
启动子：	CMV
表达水平：	高
克隆方法：	多克隆位点，限制性内切酶
载体大小：	5515 bp
5' 测序引物：	pcDNA3.1-F：CTAGAGAACCCACTGCTTAC
3' 测序引物：	pcDNA3.1-R (BGH-R)：TAGAAGGCACAGTCGAGG
载体标签：	N-His, N-EK
载体抗性：	Ampicillin (氨苄青霉素)
筛选标记：	Neomycin (G418)

质粒简介：
pcDNA3.1/His A, B, and C are 5.5 kb vectors derived from pcDNA3.1 and designed for high-level expression and purification of recombinant proteins in mammalian hosts. The vectors are supplied in three reading frames to facilitate in frame cloning with a polyhistidine metal-binding tag. The human cytomegalovirus (CMV) immediate-early promoter provides high-level expression in a wide range of mammalian cells. In addition, the vector replicates episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/His lacZ, is the pcDNA3.1/His B vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the N-terminal peptide. pcDNA3.1/His lacZ is included for use as a positive control for transfection, expression, and purification in the cell line of choice.
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