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pFastBac-GST-N质粒

  • 货号:kl-zl-1095-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-1095-01 pFastBac-GST-N质粒 NA

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pFastBac-GST-N质粒 货号:kl-zl-1095 规格:20ul

启动子: Polyhedrin
复制子: pUC ori,F1 ori
终止子: SV40 poly(A) signal
质粒分类: 昆虫细胞载体;杆状病毒组成型表达质粒
质粒大小: 5463bp
质粒标签: C-3C,C-GST
原核抗性: 氨苄青霉素Ampicillin
筛选标记: 庆大霉素Gentamicin
克隆菌株: DH5α
培养条件: 37℃,有氧,LB
表达宿主: 昆虫细胞
诱导方式: 组成型表达,无需诱导
5'测序引物: pFastbac-F: TATTCCGGATTATTCATACC
3'测序引物: pFastBac-R: ACAAATGTGGTATGGCTGA
备注: 可表达GST融合蛋白
质粒简介:
The Bac-to-Bac® Baculovirus Expression System provides a rapid and efficient method to generate recombinant baculoviruses (Ciccarone et al., 1997). This method was developed by researchers at Monsanto, and is based on site-specific transposition of an expression cassette into a baculovirus shuttle vector (bacmid) propagated in E. coli (Luckow et al., 1993). The major components of the Bac-to-Bac® Baculovirus Expression System include:
• A choice of pFastBac™ donor plasmids that allow generation of an expression construct containing the gene of interest where expression of the gene of interest is controlled by a baculovirus-specific promoter.
• An E. coli host strain, DH10Bac™, that contains a baculovirus shuttle vector (bacmid) and a helper plasmid, and allows generation of a recombinant bacmid following transposition of the pFastBac expression construct.
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