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NLS-AmCherry1-NES reporter (pDN160)哺乳荧光质粒

  • 货号：KL-ZL3333-3ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL3333-3ug NLS-AmCherry1-NES reporter (pDN160)哺乳荧光质粒 3ug

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》产品信息

货号 名称 规格
KL-ZL3333 NLS-AmCherry1-NES reporter (pDN160)哺乳荧光质粒

3ug

》产品描述

原核抗性： Amp 筛选标记： Neo/G418/mCherry 克隆菌株： DH5α 培养条件： LB/37℃

》产品简介

NLS-AmCherry1-NES reporter (pDN160)哺乳荧光质粒

》产品图谱及序列


序列：
CDS complement(5254..6114)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6115..6219)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 atggccgcag caaagcgcgt gaagctggac gtgagcaagg gcgaggagga taacatggcc
961 atcattaagg agttcatgcg cttcaaggtg cacatggagg ggtccgtgaa cggccacgtg
1021 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
1081 aaggtgacca agggcggccc cctgcccttc acctgggaca tcctgagccc tcagttcatg
1141 tacggctcca atgcctacgt gaagcacccc gccgacatcc ccgactactt taagctgtcc
1201 ttccccgagg gcttcaagtg ggagcgcgtg atgaaattcg aggacggcgg cgtggtgacc
1261 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
1321 accaacttcc cctccgacgg ccccgtgatg cagaagaaga ccatgggctg ggaggccctc
1381 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg aggtcaagcc cagagtgaag
1441 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
1501 gtgcagctgc ccggcgccta caacgtcaac cgcaagctgg acatcaccag ccacaacgag
1561 gactacacca tcgtggagca gtacgagaga gccgagggcc gccactccac cggcggcatg
1621 gacgagctgt acaagggtgg atctggaggt tcaggtggaa gtgagggagt catgctgatt
1681 aagaaaactg cagaaaatat tgacgagctg gccaaggccc tgcccgacct gaacctggac
1741 tagctcgagc ggccgccact gtgctggata tctgcagaat tccaccacac tggactagtg
1801 gatccgagct cggtaccaag cttaagttta aaccgctgat cagcctcgac tgtgccttct
1861 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
1921 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
1981 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
2041 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
2101 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
2161 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
2221 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
2281 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
2341 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
2401 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
2461 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
2521 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
2581 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
2641 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
2701 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
2761 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
2821 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
2881 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
2941 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
3001 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
3061 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
3121 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
3181 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
3241 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
3301 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
3361 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
3421 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
3481 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
3541 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
3601 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
3661 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
3721 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
3781 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
3841 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
3901 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
3961 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
4021 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
4081 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
4141 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
4201 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
4261 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
4321 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
4381 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
4441 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
4501 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
4561 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
4621 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
4681 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
4741 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
4801 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
4861 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
4921 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
4981 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt
5041 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
5101 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
5161 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
5221 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
5281 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
5341 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
5401 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
5461 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
5521 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
5581 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
5641 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
5701 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
5761 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
5821 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
5881 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
5941 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
6001 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
6061 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
6121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
6181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
6241 acctgacgtc

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