原核抗性： 氨苄青霉素Amp
筛选标记： 红色荧光蛋白mCherry，蓝色荧光蛋白TagBFP
克隆菌株： 大肠杆菌Stbl3
培养条件： 37℃
序列：
ORIGIN
1 cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
61 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
301 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
361 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
421 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
481 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
541 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
721 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
841 tcgaatggtg cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga
901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
1021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
1081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
1141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
1201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
1261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
1321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
1381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
1441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
1501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
1561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
1621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
1681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
1741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
1801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
1861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
1921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
1981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
2041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
2101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
2161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
2221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
2281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
2341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
2401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
2461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
2521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
2581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
2641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
2701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
2761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
2821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
2941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
3001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
3061 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
3121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
3181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
3421 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
3481 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
3541 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
3601 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
3661 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
3721 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
3781 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
3841 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
3901 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
3961 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
4021 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
4081 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
4141 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
4201 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
4261 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
4321 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
4381 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
4441 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
4501 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
4561 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
4621 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
4681 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
4741 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
4801 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
4861 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
4921 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
4981 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
5041 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
5101 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
5161 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
5341 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
5401 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
5461 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
5521 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
5581 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
5641 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
5701 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
5761 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
5821 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
5881 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
5941 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
6001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
6061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
6121 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
6181 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
6241 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
6301 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
6361 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
6421 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
6481 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
6541 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
6601 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca
6661 gacctggacg atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
6721 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
6781 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
6841 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
6901 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
6961 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
7021 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
7081 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
7141 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
7201 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
7261 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
7321 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
7381 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
7441 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
7501 gaacggatct cgacggtcgc caaatggcag tattcatcca caattttaaa agaaaagggg
7561 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
7621 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
7681 gcagagatcc agtttggatc gataagcttg atatcgaatt cggagcactg tcctccgaac
7741 gtcggagcac tgtcctccga acgtcggagc actgtcctcc gaacgtcgga gcactgtcct
7801 ccgaacggag catgtcctcc gaacgtcgga gcactgtcct ccgaacgact agttaggcgt
7861 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
7921 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctctcgaca
7981 ttcgttggat catggcgatg atggaggtgc agggaggccc gagtctggga caaacttgcg
8041 tcctgatcgt gatctttact gtcttgctgc agagcctttg cgtagcggtc acatatgtgt
8101 atttcactaa tgaactgaag cagatgcagg ataaatattc taagtctgga atcgcttgct
8161 tcttgaagga ggacgacagt tattgggatc ccaatgacga ggagtccatg aacagcccct
8221 gctggcaggt gaagtggcag ttgcggcaac tggtgcggaa gatgatcctg cggacaagcg
8281 aggaaacgat ttctacagtg caggaaaagc aacagaacat cagcccactg gtgagagaga
8341 ggggacccca gcgggtcgcc gcacacatta ccggaacccg cgggcgctct aataccctgt
8401 caagtcccaa ctccaagaac gagaaagccc tcggccgcaa gataaattcc tgggaatcaa
8461 gccgcagcgg gcacagtttt ctgtcaaact tgcacttgcg caatggggag ttggttatcc
8521 atgagaaggg cttctattac atctactctc aaacctactt taggtttcag gaagaaataa
8581 aagagaatac aaaaaacgac aaacagatgg tgcagtacat ctacaagtat accagttatc
8641 ccgaccctat ccttctcatg aagtcagcca ggaactcatg ctggtccaaa gacgctgagt
8701 atggactgta cagcatctac cagggcggga tctttgagct gaaggaaaat gaccgcatct
8761 ttgtatcagt gactaatgag catctcattg atatggatca cgaagcttca ttttttggag
8821 cctttctcgt cggctaagat ccttgacttg cggcccccct ctccctcccc cccccctaac
8881 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
8941 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
9001 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
9061 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
9121 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
9181 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
9241 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
9301 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
9361 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
9421 cgatgataaa tgagcgctgg cgggtccatg gtgagcaagg gcgaggagga taacatggcc
9481 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
9541 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
9601 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
9661 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
9721 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
9781 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
9841 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
9901 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
9961 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
10021 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
10081 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
10141 gacgagctgt acaagtaggg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc
10201 gcgactctag agtcgacctg cacgaggtta acgaattcta ccgggtaggg gaggcgcttt
10261 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
10321 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
10381 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
10441 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
10501 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
10561 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
10621 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
10681 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
10741 cgacctgcag cccaagctta ccatgagcga gctgattaag gagaacatgc acatgaagct
10801 gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa
10861 gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt
10921 cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac
10981 ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt
11041 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
11101 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat
11161 gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg
11221 cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa
11281 catcaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta
11341 ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca
11401 gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa
11461 ttaatgcagg catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa
11521 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
11581 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
11641 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
11701 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
11761 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
11821 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
11881 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
11941 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
12001 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
12061 cggatctccc tttgggccgc ctccccgcat
